モジュールドメイン再結合による細胞シグナル伝達フェノタイプの急速な多様化
Sergio G Peisajovich1, Joan E Garbarino, Ping Wei
1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
Science (New York, N.Y.)
|April 17, 2010
まとめ
細胞シグナル伝達タンパク質におけるドメイン再結合は,進化的イノベーションを推進する. この研究は,タンパク質ドメインを組み合わせることで,酵母の交配経路の多様性が遺伝子複製よりも大きくなり,交配効率が向上することを示しています.
科学分野:
- 分子生物学は分子生物学である.
- 進化生物学の進化生物学について
- システム生物学 システム生物学
背景:
- 細胞シグナル伝達タンパク質は,異なる触媒および調節ドメインを持つモジュール構造を有する.
- これらの生物学的モジュールの再結合は,進化的新奇性の重要な原動力であると仮定されています.
研究 の 目的:
- 細胞信号伝達経路におけるドメイン再結合から生じる現象的多様性を体系的に分析する.
- 信号応答のダイナミクスに対するドメイン再結合の影響を,他の遺伝的変異メカニズムと比較するために.
主な方法:
- イーストの交配経路から11のタンパク質を使用した66のキメリックドメイン再結合のライブラリを構築.
- 構築された再結合体におけるフェノタイプの多様性と経路応答のダイナミクスの体系的分析.
主要な成果:
- ドメイン再結合は,遺伝子複製,単一ドメイン複製,または2つのリンクされていないドメインの複製と比較して,経路応答ダイナミクスにおいてより大きな多様性を生み出しました.
- リコンビネントは,交配のフェノタイプが変化し,野生のタイプと比較して交配効率が向上したものもありました.
結論:
- 既存のタンパク質ドメイン間の新しい結合は,タンパク質ネットワークの進化において重要な役割を果たします.
- ドメイン再組み合わせは,新しい現象的行動を生成し,進化的イノベーションを推進する重要なメカニズムです.
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