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マイクロRNA165/6による細胞シグナリングは,遺伝子用量依存の根細胞の運命を指示する
Annelie Carlsbecker1, Ji-Young Lee, Christina J Roberts
1Institute of Biotechnology/Department of Biosciences, University of Helsinki, FIN-00014, Finland.
Nature
|April 23, 2010
まとめ
植物の根の発達は,細胞のコミュニケーションに依存しています. 転写因子とマイクロRNAは細胞間を移動し,適切な臓器形成のために用量に依存した方法でキセラム細胞のパターンを指示します.
科学分野:
- 植物発達生物学 植物発達生物学
- 分子植物科学は,分子植物科学である.
- 植物のオルガノゲネシス
背景:
- 植物根の放射線組織組織は保存されます.
- プロトキシレムやメタキシレムなどの血管組織のパターンは,臓器の発達に不可欠です.
- このパターンの根底にある細胞間通信メカニズムは完全に理解されていません.
研究 の 目的:
- 植物根の血管パターンにおける細胞間通信の分子メカニズムを解明する.
- シグナル伝達分子とシグナル伝達経路を特定し,キセラム細胞の型の中心的パターン化に関与する.
- 血管細胞と内皮細胞の間で位置情報がどのように交換されるかを理解する.
主な方法:
- 転写因子とマイクロRNAの細胞間移動を調査した.
- 遺伝子の活性化と標的mRNAの分解を研究するために遺伝分析を活用しました.
- 細胞運命を決定するシグナリング分子の投与量に依存する効果を調べた.
主要な成果:
- SHORT ROOT (SHR) と SCARECROW (SCR) の転写因子を含む血管シリンダーと内皮の間のクロスストークが実証されています.
- SHRは血管シリンダーから内皮に移動し,SCRを活性化します.
- SHR/SCRによるMIR165aおよびMIR166bマイクロRNAの活性化が特定され,SHR/SCRはHD-ZIPmRNAを標的とし,Xylem細胞のタイプ特異を投与量に依存した方法で制御する.
結論:
- キシレム細胞のパターンのポジティブな情報は,転写因子とマイクロRNAの方向的動きによって伝達されます.
- 転写因子によって調節される微RNAの活性差異は,キシレム細胞のタイプ特異を決定する.
- このシグナル伝達ネットワークは,精密な放射線組織と植物根の血管組織の機能を保証します.
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