RETRACTED: 小型RNA二重複体は,植物細胞間の移動式静音信号として機能する
Patrice Dunoyer1, Gregory Schott, Christophe Himber
1Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg 12 rue du Général Zimmer, 67084 Strasbourg cedex, France. patrice.dunoyer@ibmp-ulp.u-strasbg.fr
まとめ
小型の干渉RNA (siRNA) は,前駆RNAではなく,植物細胞間で移動し,RNAサイレンシングを誘発する. 非細胞自律的なRNA干渉に不可欠なこの動きは,siRNA複合体を含んでいる可能性が高い.
科学分野:
- 植物分子生物学 植物分子生物学
- 遺伝子サイレンシングメカニズム 遺伝子サイレンシングメカニズム
- RNA干渉経路によるRNA干渉経路である.
背景:
- 植物RNA干渉 (RNAi) 経路は,Dicer-like 4 (DCL4) によって処理される小さな干渉RNA (siRNAs) を利用して,Argonaute 1 (AGO1) 経由で標的RNAを静止させます.
- 植物におけるRNAiは,細胞自律ではないことが知られているが,移動信号のアイデンティティは曖昧である.
研究 の 目的:
- 植物RNA干渉経路における移動静音信号の性質を遺伝的に決定する.
- siRNAsまたはその前駆体が,細胞から細胞へのRNAiの拡散に起因する移動信号を構成するかどうかを調査する.
主な方法:
- siRNAの可動性を評価するために,DCL4機能の細胞特異的救済が利用されました.
- 信号伝送を遮断するためにRNAi運動の細胞特異的阻害を採用した.
- 物理的に追跡され,機械的に配送され,シRNA複製体をラベル付けして,その動きと機能を観察します.
主要な成果:
- 前体分子ではなく,外体および内体 siRNA が,植物細胞間の移動性静音信号として機能することを確立した.
- 標識されたsiRNA複合体の物理的な転位を実証し,これはトランスゲンRNAiの拡散を模倣した.
- 細胞から細胞への移動には,AGO1.1に結合する単一鎖のsiRNAではなく,siRNAの複合体が含まれていることを示唆する証拠を提供した.
結論:
- siRNAは,植物における非細胞自律RNA干渉を媒介する移動信号である.
- モバイルサイレンシングシグナルは,siRNAデュプレックスという形で,全身的な遺伝子サイレンシングを容易にする可能性が高い.
- この発見は,植物におけるRNAi経路のコミュニケーションの根本的な側面を明らかにしています.
関連する概念動画
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...


