NusE:NusG複合体は,転写と翻訳を結びつけています

Björn M Burmann¹, Kristian Schweimer, Xiao Luo

¹Lehrstuhl Biopolymere und Forschungszentrum für Bio-Makromoleküle, Universität Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, Germany.

Science (New York, N.Y.)

|April 24, 2010

まとめ

バクテリアの転写因子NusGは,転写と翻訳を結びつける. RhoとNuseとの結合に競争し,RhoとRhoの結合を説明する.

科学分野:

分子生物学は分子生物学である.
バクテリアの転写
タンパク質対タンパク質の相互作用

背景:

バクテリアの転写因子NusGは,Rho依存の転写終結に不可欠である.
NusGはRNAポリメラーゼと相互作用し,転写と翻訳の結合に関与する.

研究の目的:

バクテリアの転写終結におけるNusGの分子メカニズムを解明する.
NusGとRhoとNuseEの相互作用を調査する.

主な方法:

核磁気共鳴 (NMR) スペクトロスコーピーは,タンパク質とタンパク質の相互作用を研究するために使用されました.
Escherichia coli NusGのカルボキシル末端ドメインの構造分析.

主要な成果:

E. coli NusGのカルボキシル末端ドメインは,RhoまたはNuse (30Sリボソームタンパク質S10) と交互作用する.
NusGのアミノ端末ドメインはRNAポリメラーゼと結合し,そのカルボキシル端末ドメインは30Sリボソームサブユニット上のNuseと相互作用する.
NusGの結合について,NuseとRhoの間の競争が観察されました.

結論:

NusGは,転写と翻訳の機械の間の物理的なリンクとして機能します.
競争メカニズムは,Rho因子が翻訳されたトランスクリプトを終了できない理由を説明します.
NusGの役割を理解することは,バクテリアの遺伝子調節とオペロン終結に不可欠です.

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