関連する実験動画
Updated: Aug 8, 2026

09:40
Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
bovine adrenal angiotensin II型-1受容体をコードする補完DNAのクローン化と発現
K Sasaki1, Y Yamano, S Bardhan
1Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232.
Nature
|May 16, 1991
まとめ
研究者らは,牛のアンジオテンシンII受容体cDNAをクローン化することに成功した. この画期的な発見は,心臓血管および神経の調節に不可欠なアンジオテンシンII型1受容体の研究における以前の課題を克服しています.
科学分野:
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
- 薬理学 薬理学とは
背景:
- アンジオテンシンIIは,心血管,神経,電解質の輸送を調節する.
- 前回のアニオテンシンII受容体を分離する試みは,不安定性と低濃度のため失敗した.
研究 の 目的:
- アンジオテンシンII受容体の浄化における困難を克服するために.
- 牛のアンジオテンシンII受容体を特定し,特徴づけること.
主な方法:
- 牛のアンジオテンシンII受容体をコードする補完DNA (cDNA) の表現クローニング.
- COS-7細胞が受容体cDNAに感染する.
- リガンド結合測定と細胞内カルシウム/イノシトール三酸塩測定.
- メッセンジャーRNA発現を決定する北極点分析.
主要な成果:
- クローンされたcDNAは,Gタンパク質結合受容体トポロジーを持つ359アミノ酸タンパク質をコードする.
- トランスフェクトされた細胞は,アンジオテンシンIIおよび抗生物質に対する特異的な高親和性結合を示した.
- アンジオテンシンII/IIIの刺激により,細胞内Ca2+とイノシトール-1,4,5-トリスホスファートが増加した.
- この受容体は1型アンジオテンシンII受容体として知られています.
- 牛の副腎と腎臓でメッセンジャーRNA発現が検出されました.
結論:
- bovine angiotensin II型-1受容体の成功表現クローニング.
- クローンされた受容体は,アニオテンシンIIの重要なシグナル伝達経路を媒介する.
- これは,アンジオテンシンII受容体の機能と調節を研究するための新しい道を提供します.
関連する概念動画
Recombinant DNA
Overview
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Complementary DNA
Overview
Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
The Central Dogma
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
Adrenergic Receptors: β Subtype
β-adrenoceptors have varied sensitivities towards adrenaline, noradrenaline, and isoprenaline. The order of agonist potency is as follows:
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors have equal affinities for...
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors have equal affinities for...

