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Updated: Jun 13, 2026

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
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Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

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[FeFe]-ヒドロゲネーゼHクラスタの組成は,HydAの構造 ((DeltaEFG) で段階的に明らかになりました

David W Mulder1, Eric S Boyd, Ranjana Sarma

  • 1Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, Montana 59717, USA.

Nature
|April 27, 2010
PubMed
まとめ

この研究は,[FeFe]-ヒドロゲナース (HydA) 酵素が活性部位を組み立てる方法を明らかにしています. [4Fe-4S]クラスターが最初に挿入され,2Feサブクラスターが特殊な熟成機械を介してそれに続くことが示されています.

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