トランスメブラン受容体DCCはタンパク質合成機構と関連し,翻訳を調節する

Joseph Tcherkezian¹, Perry A Brittis, Franziska Thomas

¹Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.

Cell

|May 4, 2010

まとめ

DCCのような細胞表面受容体は,局所的にタンパク質合成を制御することができます. ネトリンなどの細胞外信号は,この受容体を活性化し,特定のニューロン領域の翻訳を調節します.

科学分野:

神経科学は神経科学である.
分子生物学は分子生物学である.
細胞生物学細胞生物学

背景:

細胞外信号は,細胞機能にとって極めて重要なタンパク質翻訳を調節する.
空間的にタンパク質合成を制御することで,細胞のサブ領域内の正確な調節が可能になります.
細胞外信号と空間的に正確な翻訳を結びつけるメカニズムは,ほとんど不明のままである.

研究の目的:

細胞外線を空間的に正確なタンパク質翻訳に結びつけるメカニズムを調査する.
局所タンパク質合成の調節におけるトランスメブラン受容体の新たな役割を特定する.

主な方法:

共同免疫プレシピテーションは,DCCの結合パートナーを特定するための分析である.
免疫光顕微鏡で,翻訳機械によるDCCの局所化を視覚化します.
神経細胞のコンパートメントで新たに合成されたタンパク質の分析.

主要な成果:

結腸直腸がん (DCC) の削除された受容体は,ユカリオット発現因子とリボソームを含む翻訳機構の構成要素と複合体を形成します.
DCCは,ニューロン軸索とデンドライトの翻訳機構と新たに合成されたタンパク質とコロカライズします.
細胞外リガンド・ネットリンは,DCC媒介による翻訳と翻訳成分の解離を促進する.

結論:

細胞表面受容体 (DCC) は,物理的にも機能的にも,タンパク質翻訳機構と関連しています.
この結合はトランスメブラン翻訳調節複合体を形成し,タンパク質合成の局所的な細胞外制御を可能にします.
これは,細胞外信号によるタンパク質合成の空間的調節のための一般化可能なモデルを提供します.

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