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Updated: Jul 21, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

溶液中のより大きなタンパク質の構造: 3次元と4次元ヘテロ核性NMRスペクトロスコピー

G M Clore1, A M Gronenborn

  • 1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

Science (New York, N.Y.)
|June 7, 1991
PubMed
まとめ

3次元および4次元ヘテロ核核磁気共振 (NMR) スペクトロスコピーは,タンパク質構造の決定のためのスペクトル解像度を高めます. この高度なNMR技術により,より大きなタンパク質の分析が可能になり,150個の残基を超えて拡大できます.

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¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

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¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

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Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
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2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

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2D NMR: Overview of Heteronuclear Correlation Techniques

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