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NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
NMR and Mass Spectroscopy of Carboxylic Acids01:30

NMR and Mass Spectroscopy of Carboxylic Acids

In ¹H NMR spectroscopy, acidic protons (–COOH) of carboxylic acids are highly deshielded and absorb far downfield, at around 9–12 ppm. The chemical shift value depends on the concentration and solvent used.
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the disappearance of the acidic...
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...

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Updated: Jun 12, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

タンパク質のオリエンテッドサンプルの固体NMRのための共振割り当て方法.

Robert W Knox1, George J Lu, Stanley J Opella

  • 1Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, Raleigh, North Carolina 27695-8204, USA.

Journal of the American Chemical Society
|June 1, 2010
PubMed
まとめ
この要約は機械生成です。

この研究は,均一に窒素-15で標識された膜タンパク質の固体状態のNMRスペクトルを割り当てる新しい方法を導入しています. この技術は,既存の割り当てを確認し,特殊なラベル付けなしにタンパク質の構造を決定するのに役立ちます.

さらに関連する動画

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

関連する実験動画

Last Updated: Jun 12, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

科学分野:

  • 構造生物学 構造生物学とは
  • バイオフィジックス 生物物理学
  • 核磁共振 (NMR) スペクトロスコピー

背景:

  • 膜タンパク質の固体NMRスペクトルを割り当てることは,構造の決定に不可欠です.
  • 単軸並べられたタンパク質は,ユニークな洞察力を提供しますが,割り当ての課題を提起します.
  • 既存の方法は,選択的に標識されたサンプルを必要とし,広範な適用性を制限しています.

研究 の 目的:

  • 均一に (15) N-ラベルを貼った単軸に並べられた膜タンパク質のための一般的な順序的配分戦略を提案する.
  • 磁気的に調整されたPf1ファグコートタンパク質を用いて,方法の有効性を実証する.
  • 固体状態NMRによる膜タンパク質の構造決定を容易にする.

主な方法:

  • 不一致したハートマン・ハーン磁気化移転を用いて,15Nの脊柱回転の間の陽子媒介の相関を証明する.
  • 交換されたおよび交換されていない分離されたローカルフィールドスペクトルの取得およびオーバーレイ.
  • 単軸配列の膜タンパク質,特にPf1ファグのコートタンパク質を使用しています.

主要な成果:

  • 選択的にラベル付けされたサンプルを必要とせずに,文献からほとんどのオリジナルの割り当てを成功裏に確認しました.
  • 異なるスペクトル取得を比較することによって,クロスピークとメインピークを区別する.
  • 任意のトポロジーのタンパク質に適用できることを実証した.

結論:

  • 提案された順次配分戦略は,均一に (15) N-ラベルをつけられた単軸に並べられた膜タンパク質に有効です.
  • この方法はスペクトルの割り当てを簡素化し,複雑なラベリング戦略の必要性を軽減します.
  • この技術は,将来の固体状態のNMRベースの膜タンパク質の構造決定に価値があります.