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関連する概念動画

Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...

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Updated: May 7, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

ヌクレオソームの位置とDNAメチレーションの関係

Ramakrishna K Chodavarapu1, Suhua Feng, Yana V Bernatavichute

  • 1Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California 90095, USA.

Nature
|June 1, 2010
PubMed
まとめ

アラビドプシス・タリアナの全ゲノムにわたる核細胞の位置づけは,核細胞がDNAメチル化パターンを影響することを明らかにしています. この保存されたメカニズムは,DNAメチルトランスファーゼが核細胞結合DNAを標的とし,遺伝子調節に影響を及ぼすことを示唆しています.

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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

関連する実験動画

Last Updated: May 7, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

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科学分野:

  • 分子生物学は分子生物学である.
  • エピジェネティクス エピジェネティクス
  • ゲノミクスゲノミクスとは

背景:

  • DNAとヒストンのオクトーマーで構成される核細胞は,核内のDNA組織と調節の基本的な単位です.
  • ヌクレオソームの位置づけを理解することは,ゲノムアクセシビリティと遺伝子発現制御の解読に不可欠です.

研究 の 目的:

  • アラビドプシス・タライアナのニュクレオソームの位置づけに関する全ゲノム分析を行うために.
  • 核細胞の位置とDNAメチル化パターンとの関係を調査する.
  • これらの関係がヒトのDNAに保存される様子を調査する.

主な方法:

  • アラビドプシス・タリアナからモノヌクレオソームを大量に並行的に配列化する.
  • 核細胞位置データと単塩基解像度のDNAメチル化プロファイルを統合する.
  • 人間の核染色体DNAデータとの比較分析.

主要な成果:

  • 核細胞結合DNA内のDNAメチル化における10塩基周期性を特定した.
  • ヌクレオソームのDNAは,隣接するDNAよりもメチル化されていることが示されました.
  • 保存されたメカニズムを示唆するヒト核細胞DNAの保存された傾向を観察した.
  • エクソンとイントロン-エクソン境界の核細胞濃縮が発見され,動物研究と類似している.
  • エクソンにおけるRNAポリメラーゼIIとDNAメチル化の濃縮が認められ,遺伝子調節とエクソン定義における役割を示唆している.

結論:

  • 核子の位置は,ゲノム全体のDNAメチル化パターンに大きく影響する.
  • DNAメチルトランスフェラーゼは,核細胞結合DNAを優先的に標的にする.
  • ヌクレオソームとDNAメチレーションの相互作用は,種を超えて保存されています.
  • 核子の位置づけとDNAメチル化は,RNAポリメラーゼIIのプロセシビティとエクソン定義の調節に役割を果たす可能性があります.