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Updated: Jun 12, 2026

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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繰り返しの飽和変異は,酵素のステレオ選択性の実験室での進化を加速する:伝統的な方法との厳格な比較

Manfred T Reetz1, Shreenath Prasad, José D Carballeira

  • 1Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr, Germany. reetz@mpi-muelheim.mpg.de

Journal of the American Chemical Society
|June 12, 2010
PubMed
まとめ

繰り返し飽和変異 (ISM) は,ステレオ選択性および安定性のための酵素進化を大幅に強化します. この方法は,Pseudomonas aeruginosa lipaseの誘導進化のための以前の技術よりも効率的であることが示されました.

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