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Updated: Jan 17, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
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High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

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生物学的固体内の動的領域の割り当ては,スピン状態の選択的NMR実験によって可能になりました

Rasmus Linser1, Uwe Fink, Bernd Reif

  • 1Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-St. 10, 13125 Berlin-Buch, Germany.

Journal of the American Chemical Society
|June 12, 2010
PubMed
まとめ

タンパク質の間の時間スケールの動きは,固体状態のNMR分析を妨げます. トリプル共振実験における横断リラクゼーション最適化スペクトルスコピー (TROSY) 技術は,これを克服し,ダイナミックなタンパク質領域の詳細な構造分析を可能にすることを実証しました.

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The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
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