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関連する概念動画

Mitosis and Cytokinesis02:03

Mitosis and Cytokinesis

221.8K
In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
221.8K
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

2.1K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
2.1K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.9K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.9K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.5K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

5.4K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.4K
Mitosis and Cytokinesis01:35

Mitosis and Cytokinesis

9.9K
In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
9.9K

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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

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メタゾアの核毛孔複合体の組成における細胞周期に依存する差異.

Christine M Doucet1, Jessica A Talamas, Martin W Hetzer

  • 1Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, 10010 North Torrey Pines Road, La Jolla, 92037 CA, USA.

Cell
|June 17, 2010
PubMed
まとめ

核孔複合体 (NPC) は,ミトーシスとインターフェーズの間に異なる組み立てメカニズムを使用します. ELYSはミトーシス後のNPC形成を促進し,POM121はインターフェーズNPC組立に不可欠である.

科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー

背景:

  • 核孔複合体 (NPC) は,核と細胞質の間の輸送を媒介する.
  • NPCはミトーシスとインターフェーズの両方で核封筒 (NE) に組み合わさなければなりません.
  • 独特の細胞イベントとトポロジカルな制約は,NEの再生と成長の間に存在します.

研究 の 目的:

  • 細胞周期の異なる段階におけるNPC組立中のヌクレオポリンに対する差異的な要求を調査する.
  • オープンミトーシスとインターフェーズの核膜トポロジーの文脈で,NPCアセンブリを制御するメカニズムを解明する.

主な方法:

  • 特定のヌクレオポリン (ELYS,POM121) のRNAi媒介によるノックダウン.
  • クロマチンと核封筒へのNup107/160複合体のリクルートメントの分析.
  • NPCアセンブリにおけるNup133の膜曲線感知ドメインの役割に関する調査.

主要な成果:

  • ELYSは,ミトーシス後のNPCアセンブリに不可欠ですが,インターフェーズでは必要ありません.
  • POM121は,Nup107/160複合体をインターフェーズ中の新しい組み立て現場に組み込むために必要です.

さらに関連する動画

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

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Last Updated: May 5, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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18.3K
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

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  • Nup133の膜曲線感知ドメインは,インターフェーズのNPCアセンブリには重要ですが,ミトスの後のNPC形成には重要ではありません.

    • 結論:

    • メタゾーンNPCは,細胞サイクル依存のNEトポロジーに合わせた少なくとも2つの異なるアセンブリメカニズムを使用します.
    • ELYSとPOM121は,それぞれミトーシスとインターフェーズの間に,NPCアセンブリにおいて,重複しない特定の役割を担っています.
    • この発見は,核包膜の完全性にとって極めて重要なNPC生殖における細胞サイクル特有の適応を明らかにしている.