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[FeFe]-ヒドロゲナーゼ成熟:HydG触媒による一酸化炭素の合成
Eric M Shepard1, Benjamin R Duffus, Simon J George
1Department of Chemistry & Biochemistry and Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, Montana 59717, USA.
Journal of the American Chemical Society
|June 23, 2010
まとめ
酵素HydGはチロシンから一酸化炭素 (CO) を合成し,これは[FeFe]-ヒドロゲネーゼの成熟における重要なステップである. これは,ヒドロゲンアースバイオシンセシスにおける酵素によるCO生成の最初の実証である.
科学分野:
- バイオケミストリー バイオケミストリー
- 酵素学 酵素学とは
- 有機金属化学 有機金属化学
背景:
- [FeFe]-ヒドロゲネーゼの活性部位には,有機金属のH-クラスターが含まれています.
- その生物合成には,HydE,HydG (過激なAdoMet酵素),HydF (GTPase) の付属タンパク質が必要です.
- 一酸化炭素 (CO) は,ヒドロゲネーゼ活性部位 ([FeFe],[NiFe],[Fe]のみ) の一般的なリガンドである.
研究 の 目的:
- ハイドロゲンアース活性部位バイオシンセシスにおけるHydGの役割を調査する.
- HydGが一酸化炭素 (CO) の合成を触媒化するかどうかを判断する.
- HydGによるCO生成のための基質を特定する.
主な方法:
- CO生成を検出するレポーターとしてデオキシヘモグロビンを利用しました.
- 可視光譜検査でカルボキシヘモグロビン (HbCO) の形成をモニターした.
- COの起源を確認するために使用された (13) C-チロシンとフーリエ変換赤外線スペクトロスコーピー (FTIR).
主要な成果:
- HydGがCOの合成を触媒として作用することを実証した.
- HydG.によるCO生成の基質としてチロシンが確認された.
- ハイドロゲンアースの成熟過程における酵素によるCO合成の最初の証拠を提供した.
結論:
- HydGはチロシンからのCOの酵素合成を担当しています.
- この発見は,[FeFe]-ヒドロゲネーゼの複雑な成熟経路における重要なステップを明らかにしています.
- ハイドロゲネーゼ機能に関連するCO生成のための新しい酵素経路を確立します.
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