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Updated: Jun 12, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

生体細胞表面にタグ融合したGPCRタンパク質を,その機能分析のための合成探査機で選択的共性ラベリングする

Hiroshi Nonaka1, Sho-hei Fujishima, Sho-hei Uchinomiya

  • 1Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto 615-8510, Japan.

Journal of the American Chemical Society
|June 24, 2010
PubMed
まとめ

研究者らは,細胞表面上のGタンパク質結合受容体 (GPCRs) のための新しい共性ラベリング方法を開発しました. このテクニックは,ペプチドタグと機能化された分子プローブを使用して,生体細胞における迅速で選択的なタンパク質のラベル付けと視覚化を行います.

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