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Updated: Jun 11, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

マウスの生殖系における全ゲノム再プログラミングは,塩基切除修復経路を伴う

Petra Hajkova1,2, Sean J Jeffries1, Caroline Lee1

  • 1Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK.

Science (New York, N.Y.)
|July 3, 2010
PubMed
まとめ

マウスの原始生殖細胞 (PGC) の活性DNA脱メチル化は,エピジェネティクスとトーティポテンシーの再プログラムに不可欠なDNA断裂と塩基切除修復 (BER) を含む.

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
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