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関連する概念動画

Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

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関連する実験動画

Updated: Jun 11, 2026

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

ヒストン改変複合体のモジュール構造の支架として,ロングノンコーディングRNA.

Miao-Chih Tsai1, Ohad Manor, Yue Wan

  • 1Howard Hughes Medical Institute and Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Science (New York, N.Y.)
|July 10, 2010
PubMed
まとめ

長いインタージェニック・ノンコーディングRNA (lincRNAs) は,異なるヒストン改変複合体を結合して,支架として作用する. このRNA媒介アセンブリは,クロマチンの特定のヒストン改変を誘導することによって,表遺伝子遺伝を調整する.

さらに関連する動画

RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

関連する実験動画

Last Updated: Jun 11, 2026

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

科学分野:

  • 分子生物学は分子生物学である.
  • エピジェネティクス エピジェネティクス
  • ゲノミクスゲノミクスとは

背景:

  • ロングインタージェニック・ノンコーディングRNAs (lincRNAs) は,クロマチンの状態と表遺伝子遺伝の重要な調節体である.
  • lincRNAsが遺伝子発現に影響を与えるメカニズムを理解することは,表遺伝的調節を解読するために重要です.

研究 の 目的:

  • ヒストン改変複合体の支架としてlincRNAHOTAIRの役割を調査する.
  • HOTAIRが,クロマチンに特異な表皮遺伝的変形剤を組み立て,標的化することをどのように促進するか解明する.

主な方法:

  • HOTAIRとヒストン改変複合体間の結合相互作用を決定するための生化学的分析.
  • クロマチン免疫プレシピテーション (ChIP) は,複合体の特定のゲノム位置へのターゲティングを評価します.

主要な成果:

  • HOTAIRは,ポリコンブ・レプレッシブ・コンプレックス2 (PRC2) をその5'ドメインと,LSD1/CoREST/REST複合体をその3'ドメインで結合させ,支架として機能する.
  • HOTAIRによるRNA媒介によるPRC2とLSD1の組み立てにより,調整されたヒストンH3ライシン27メチル化とライシン4脱メチル化が可能になります.
  • この協調的な行動は,標的遺伝子のヒストン改変のパターンを指定します.

結論:

  • HOTAIRによって例示される lincRNAs は,ヒストン改変酵素を組み立てるための支架として機能することができます.
  • この脚本メカニズムは,RNA媒介による染色体状態の調節と表遺伝的継承のための新しい経路を提供します.
  • この発見は,表遺伝的修正を特定する際のリンカRNA機能の一般的な原理を強調しています.