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Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Deep Sea Microbial Ecology01:18

Deep Sea Microbial Ecology

The deep ocean and its underlying sediments represent vast, largely unexplored microbial habitats that extend far beyond the sunlit photic zone. The photic (euphotic) zone typically spans the upper ~100–200 meters of pelagic waters in the open ocean, but its depth varies geographically and seasonally, where sufficient light supports photosynthetic life. Below this lies the deep sea, spanning roughly 1000–6000 meters (bathypelagic to abyssal zones), with deeper hadal trenches extending beyond...
Microbes and Other Elemental Cycles01:24

Microbes and Other Elemental Cycles

Microbial activity plays a pivotal role in the biogeochemical cycling of iron and manganese, especially at the redox gradients characteristic of stratified aquatic environments. These cycles are driven by microbial transformations between oxidized and reduced forms of the metals, allowing organisms to exploit them for metabolic energy and structural purposes.Iron Cycling Across Redox GradientsIn neutral, oxygen-rich surface waters, iron is predominantly found in its oxidized, insoluble ferric...
Methods to Assess Microbial Communities01:19

Methods to Assess Microbial Communities

Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
Microbial Corrosion01:24

Microbial Corrosion

Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...
Microbial Nutrition01:28

Microbial Nutrition

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微生物のメタロプロテオームは,大部分が特徴づけられていない.

Aleksandar Cvetkovic1, Angeli Lal Menon, Michael P Thorgersen

  • 1Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA.

Nature
|July 20, 2010
PubMed
まとめ

この研究は,生物が使用するすべての金属とその金属タンパク質を特定するための新しい金属ベースの方法を導入しています. 研究は,メタロプロテオームがこれまで理解していたよりも広範囲で多様で,細胞生物学と毒性メカニズムに影響を及ぼすことを明らかにしています.

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Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
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Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

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Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
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Published on: April 13, 2016

科学分野:

  • バイオケミストリーと分子生物学
  • 微生物学 微生物学とは
  • プロテオミクス プロテオミクスは,プロテオミクスの

背景:

  • メタロプロテインは,メタルイオン共因子を触媒,電子移転,および安定性のために利用し,生物学的プロセスに不可欠です.
  • 現在の方法は,多様で認識されていない金属の協調部位により,生物の金属タンパク質を予測するのに苦労しています.
  • メタロプロテオームを理解することは,細胞生物学,微生物の成長,および毒性についての洞察にとって不可欠です.

研究 の 目的:

  • 同化金属および金属タンパク質の全ゲノム識別のための堅固な金属ベースのアプローチを開発し,検証する.
  • *Pyrococcus furiosus*のサイトプラズマメタルプロテオームを特徴付け,他の微生物と比較する.
  • メタロプロテオームの全範囲と多様性を明らかにする.

主な方法:

  • 液体クロマトグラフィを用いた金属ベースの識別と浄化.
  • タンパク質の識別のための高通量タンデム質量スペクトロメトリー (HT-MS/MS).
  • 金属の定量化のための誘導結合プラズマ質量スペクトロメトリー (ICP-MS).

主要な成果:

  • 金属ベースの戦略では,3つの微生物 (*Pyrococcus furiosus*, *Escherichia coli*, *Sulfolobus solfataricus*) の158の予期せぬ金属タンパク質が特定されました.
  • 鉛,マンガネス,モリブデン,ウラン,バナジウムを含む予期せぬ金属が同化されていることが判明しました.
  • 新種のニッケルとモリブデンを含むタンパク質が発見され,鉛とウランが誤って組み込まれたタンパク質も発見されました.

結論:

  • メタロプロテオームは,以前に認識されたものよりもはるかに広大で多様です.
  • 開発された金属ベースのアプローチは,包括的なメタロプロテオーム分析のための強力なツールを提供します.
  • 発見は,微生物の生理学,金属の吸収,および毒性のメカニズムに関する重要な洞察を提供します.