Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Predicting Patient Status in Chronic Thromboembolic Pulmonary Hypertension Using a Biophysical Model.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2023
Same author

Blunt bronchial injury management with veno-venous extracorporeal membrane oxygenation providing a peri-operative 'survival bridge'.

Trauma case reports·2020
Same author

Detection of haplotype-dependent allele-specific DNA methylation in WGBS data.

Nature communications·2020
Same author

Autoantibodies to killer cell immunoglobulin-like receptor 3DL1 in patients with systemic lupus erythematosus.

Clinical and experimental immunology·2018
Same author

Clinical and clinicopathological characteristics of acute lymphoblastic leukaemia in six cats.

The Journal of small animal practice·2018
Same author

Shorter duration of antibiotic treatment for acute bacteraemic cholangitis with successful biliary drainage: a retrospective cohort study.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2018

関連する実験動画

Updated: Jun 10, 2026

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
12:08

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Published on: March 10, 2016

誘導された多能幹幹細胞における表遺伝子記憶

K Kim1, A Doi, B Wen

  • 1Stem Cell Transplantation Program, Division of Pediatric Hematology/Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA

Nature
|July 21, 2010
PubMed
まとめ

ソマティック細胞核移転 (SCNT) は,多能性を達成するために,転写因子ベースの再プログラミングよりも効果的です. SCNT由来細胞はエピジェネティックメモリが少ないため,誘発性多能幹細胞 (iPSC) に比べてより広い差別化潜在能力を発揮します.

さらに関連する動画

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
07:46

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Published on: July 28, 2023

関連する実験動画

Last Updated: Jun 10, 2026

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
12:08

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Published on: March 10, 2016

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
07:46

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Published on: July 28, 2023

科学分野:

  • エピジェネティクス エピジェネティクス
  • 幹細胞生物学 幹細胞生物学
  • 発達生物学 発達生物学について

背景:

  • 体細胞核移転 (SCNT) と転写因子ベースの再プログラミングは,成人細胞から多能幹細胞を生成する方法である.
  • この2つの方法とも,遺伝子の発現に影響を与えるエピジェネティック変異であるゲノムメチレーションをリセットします.
  • 再プログラムメカニズムの違いは,その結果生じる多能幹細胞の性質の潜在的な変化を示唆する.

研究 の 目的:

  • SCNT由来および誘発性多能幹細胞 (iPSCs) の間における表遺伝的状態と微分の可能性の違いを調査する.
  • 起源の体組織からの表遺伝的記憶が,iPSCの性質に影響するかどうかを決定する.
  • プラリポテンシーの基本状態の確立におけるSCNTと因数ベースの再プログラミングの有効性を比較する.

主な方法:

  • 低通路のiPSCとSCNT由来多能幹細胞におけるDNAメチル化パターンの比較.
  • 様々な細胞系に沿った微分化の可能性の評価.
  • エピジェネティックメモリリセットのリセットを調査するために,クロマチンを改変する薬剤でiPSCの治療.

主要な成果:

  • iPSCは,その起源の体組織から残ったDNAメチル化シグネチャを保持し,ドナー特異的な差異化を促進します.
  • iPSCのこの"表遺伝子記憶"は,他の細胞の運命を制限しますが,リセットすることができます.
  • SCNT由来の多能幹細胞は,iPSCよりも胚性幹細胞に似ている差別化およびメチル化パターンを示す.

結論:

  • SCNTは,多能性の基本状態を確立する上で,因数ベースの再プログラミングよりも効果的です.
  • 要素ベースの再プログラミングは,指向された差異化に影響を与えるエピジェネティックメモリを残すことができます.
  • これらのエピジェネティック的な違いを理解することは,疾患モデリングと再生医療の応用に不可欠です.