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タンパク質のS-ニトロシル化およびS-グルタチオニル化部位を特定するための金ナノ粒子濃縮法

Adam Faccenda1, Christopher A Bonham, Panayiotis O Vacratsis

  • 1Department of Chemistry & Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada.

Journal of the American Chemical Society
|August 4, 2010
PubMed
まとめ

ゴールドナノ粒子 (AuNPs) は,タンパク質S改変部位を特定するために,チオール改変ペプチドを分離し,濃縮するための簡単な方法を提供します. このテクニックは,感受性の高いチオルを割り当て,プロテオミク解析を進めるのに役立ちます.

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Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.