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関連する概念動画

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

哺乳類のマイクロRNAは,主に標的mRNAのレベルを低下させる作用をします.

Huili Guo1, Nicholas T Ingolia, Jonathan S Weissman

  • 1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.

Nature
|August 13, 2010
PubMed
まとめ
この要約は機械生成です。

マイクロRNA (miRNA) は,主に,トランスレーションを阻害することによってではなく,標的メッセンジャーRNA (mRNA) レベルを下げることによって,タンパク質の生産を減少させます. この発見は,miRNA媒介による遺伝子調節の主なメカニズムとしてmRNAの不安定化を強調しています.

さらに関連する動画

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

関連する実験動画

Last Updated: Jun 10, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • 遺伝子規制 遺伝子規制

背景:

  • マイクロRNA (miRNA) は,遺伝子発現を調節する小さなノンコーディングRNAです.
  • miRNA媒介の遺伝子抑制は,翻訳的阻害またはmRNAの劣化によって起こる可能性があります.
  • これらのメカニズムの相対的な貢献は,特に内生的なターゲットの場合は,ほとんど特徴づけられていないままです.

研究 の 目的:

  • 翻訳抑制とmRNAの分解がmiRNA媒介のタンパク質生産の減少に与える影響を定量化する.
  • エクトピックおよび内生的な miRNA 標的の両方のこれらの効果を比較するために.
  • miRNAsがタンパク質の出力を調節する主要なメカニズムを解明する.

主な方法:

  • 全球タンパク質合成速度を測定するためにリボソームプロファイリングを使用しました.
  • 同時にメッセンジャーRNA (mRNA) レベルの変化を評価した.
  • エクトピー的に発現したおよび内生的に調節されたmiRNA標的の両方を分析しました.

主要な成果:

  • 減少したmRNAレベルは,子宮外および内生的なmiRNA相互作用の両方の減少したタンパク質生産の大部分 (>=84%) を占めた.
  • タンパク質の生産量に対するmiRNA調節の影響は,mRNA豊富さの変化と密接に相関していた.
  • mRNAの不安定化は,miRNA誘発のタンパク質減少の主な原動力として特定されました.

結論:

  • miRNA媒介による遺伝子サイレンシングは,主に標的mRNAの不安定化によって発生します.
  • mRNAレベルの変化は,miRNAの規制効果の信頼できる指標として機能します.
  • このメカニズムを理解することは,遺伝子調節を理解し,miRNAベースの治療法を開発するために不可欠です.