関連する実験動画
Updated: Jun 7, 2026
11:32
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Published on: May 24, 2017
酵母におけるRNA二次構造の全ゲノム測定
Michael Kertesz1, Yue Wan2, Elad Mazor1
1Dept. of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.
Nature
|September 3, 2010
まとめ
新しい方法であるRNA構造の並列分析 (PARS) は,RNAの二次構造を全ゲノムで測定する. この技術は,酵母メッセンジャーRNA (mRNA) の重要な構造的性質とその翻訳効率との関係性を明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- ゲノミクスゲノミクスとは
- バイオケミストリー バイオケミストリー
背景:
- RNAの構造は,分子機能と調節に不可欠です.
- 既存の実験技術は,ゲノムスケールのRNA構造測定能力が欠けている.
研究 の 目的:
- ゲノムスケールRNA構造プロファイリングのための新しい方法を導入する.
- Saccharomyces cerevisiaeにおけるメッセンジャーRNA (mRNA) の二次構造を調査する.
主な方法:
- RNA構造の並列分析 (PARS) を開発し,適用しました.
- PARSは,構造特異な酵素と,RNA断片の深層配列化を活用しています.
- 何千ものRNA種を同時に単一のヌクレオチド解像度で解析した.
主要な成果:
- 3,000以上の酵母 mRNA トランスクリプトの二次構造を成功裏にプロファイリングしました.
- 翻訳されていない地域と比較して,コーディング地域における二次構造の増加を特定しました.
- コーディング領域の二次構造に3つの核酸の周期性が見られた.
- 翻訳開始地点でのmRNA翻訳効率と構造との間には逆相関が認められた.
結論:
- PARSは,ゲノム規模のRNA構造分析のための強力なツールです.
- イーストのmRNAは,その機能に関連した独特の構造的特性を示す.
- PARSは,RNA構造のダイナミクスを研究するために,多様な生物と条件に適用することができます.
関連する概念動画
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
03:21RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
01:19RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...