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関連する概念動画

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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関連する実験動画

Updated: Jun 8, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

CRISPR内核酵素による配列および構造特異的なRNA処理.

Rachel E Haurwitz1, Martin Jinek, Blake Wiedenheft

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

Science (New York, N.Y.)
|September 11, 2010
PubMed
まとめ
この要約は機械生成です。

研究者は,Pseudomonas aeruginosa.でCRISPRトランスクリプト (pre-crRNAs) の処理に不可欠なCsy4酵素を特定しました. この酵素は,選択的結合と分裂のために特定のRNA相互作用と保存された残基を使用し,CRISPR免疫システム機能を説明します.

さらに関連する動画

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

関連する実験動画

Last Updated: Jun 8, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

科学分野:

  • 微生物学 微生物学とは
  • 分子生物学は分子生物学である.
  • 構造生物学 構造生物学とは

背景:

  • バクテリアと古生物は,外来遺伝子要素に対する適応免疫のために,クラスター化された規則的に間隔の短いパリンドローム繰り返し (CRISPRs) を利用します.
  • CRISPR媒介免疫は,CRISPRロシのトランスクリプトから生成されたCRISPR由来RNA (crRNAs) に依存しています.

研究 の 目的:

  • Pseudomonas aeruginosaのCRISPRトランスクリプト (pre-crRNAs) の処理を担当する特定のエンドロビヌクレアスを特定する.
  • 特定された酵素によるcrRNA前処理の分子メカニズムを解明する.

主な方法:

  • 細菌遺伝学では,Pseudomonas aeruginosaの酵素を特定する.
  • RNAに結合した酵素の1.8アングストロムの結晶構造を決定するX線結晶学.
  • RNA認識と分裂メカニズムを分析するための生化学分析.

主要な成果:

  • エンドロビヌクレアースCsy4は,Pseudomonas aeruginosa.のcrRNA前処理を担当する酵素として特定されました.
  • 構造分析は,Csy4とcrRNAの重複幹ループの間の配列特異的な相互作用をメジャー・グリューブで明らかにした.
  • Csy4は,その活性部位に保存されたセリンおよびヒスティジン残留物を利用して,前crRNAsの選択的結合と分裂を行います.

結論:

  • この研究は,Csy4.4による配列および構造特異的なcrRNA前処理のメカニズムを明らかにしています.
  • 特定されたRNA認識メカニズムは,CRISPR特異性endoribonucleasesの広範なファミリーの機能についての洞察を提供します.
  • Csy4の機能を理解することは,細菌の適応免疫とCRISPR-RNAの生体生成を理解するのに役立ちます.