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関連する概念動画

Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mismatch Repair01:36

Mismatch Repair

Overview

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関連する実験動画

Updated: Jun 8, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

デュプレックスDNAとトリプレックスDNAの非ヌクレオチドループ置換の構造最適化

Squire Rumney1, Eric T Kool

  • 1Department of Chemistry, University of Rochester, Rochester, New York 14627.

Journal of the American Chemical Society
|September 28, 2010
PubMed
まとめ
この要約は機械生成です。

エチレングリコルのオリゴーマーがDNAのデュプレックスとトリプレックスを安定させる. 予想以上に長く,最適化されたリンクは,診断プローブと治療薬の熱安定性を高めます.

さらに関連する動画

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

関連する実験動画

Last Updated: Jun 8, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

科学分野:

  • 合成生物学 合成生物学とは
  • 核酸化学について
  • 生物物理化学 生物物理化学とは

背景:

  • ヌクレオチドループは,DNAの構造と機能に不可欠です.
  • 既存の非ヌクレオチドループの交換には,安定性の限界があります.
  • エチレングリコール (EG) オリゴーマーは,DNAリンクナーを安定させる可能性がある.

研究 の 目的:

  • DNAにおける非ヌクレオチドループ置換体としてのエチレングリコール (EG) オリゴマーの構造的効果を調査する.
  • 新しく,構造的に最適化されたEGベースのリンカーを合成し,特徴づけること.
  • DNAのデュプレックスとトリプレックスにおけるこれらのリンカーの安定効果を評価する.

主な方法:

  • 自動DNA合成のために,フォスフォラミジートとして誘導されたEGオリゴマーの合成.
  • EGリンクヤーをDNAのデュプレックスとトリプレックス配列に組み込む.
  • ヘリックス安定性を測定するための熱変性 (Tm) 分析.

主要な成果:

  • ヘプタキス (エチレングリコール) リンクは,自然T (4) ループの性能を上回り,デュプレックスで最大の熱安定性を提供しました.
  • オクタキス ((エチレングリコール)) (EG ((8)) リンクは,様々な方向とターゲットストランドの長さでトリプレックスで最も高い安定性を示しました.
  • EG(8) 結合されたストランドは,特定のトリプルクス構成で,天然のT(5) ループに匹敵する,またはそれを上回る結合アフィニティを示した.

結論:

  • 最適化されたエチレングリコールオリゴーマーが,DNAの非核酸回路置換を効果的に安定させることに役立ちます.
  • 最適な長さは,単純な幾何学的な予測を超えています.
  • これらの発見は,診断,研究,治療のための合成核酸の設計に価値があります.