Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Contact-dependent Signaling01:19

Contact-dependent Signaling

Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Anchoring Junctions01:03

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Mechanoreceptor plexin D1 regulates lymphatic valve morphogenesis and lymphedema pathogenesis.

The Journal of clinical investigation·2026
Same author

Design principles of a membrane-spanning ubiquitin ligase.

Molecular cell·2026
Same author

Pace of ecology drives the tempo of visual perception across the animal kingdom.

Nature ecology & evolution·2026
Same author

Preclinical assessment of two FcγRI-specific antibodies that competitively inhibit immune complex-FcγRI binding to suppress autoimmune responses.

Nature communications·2025
Same author

Conceptual and methodological flaws undermine claims of a link between the gut microbiome and autism.

Neuron·2025
Same author

The E3 ubiquitin ligase MGRN1 targets melanocortin receptors MC1R and MC4R via interactions with transmembrane adapters.

Journal of cell science·2025

関連する実験動画

Updated: May 13, 2026

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay
12:52

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay

Published on: March 4, 2011

セマフォリン-プレキシンの信号伝達の構造的基礎

Bert J C Janssen1, Ross A Robinson, Francesc Pérez-Brangulí

  • 1Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK.

Nature
|September 30, 2010
PubMed
まとめ

セマフォリン-プレキシン細胞の信号伝達は,2:2複合体による. この研究は,セマフォリン-プレキシン相互作用の構造的基礎を明らかにし,発達と疾患におけるシグナル伝達機構を明らかにします.

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 構造生物学 構造生物学とは

背景:

  • セマフォリンリガンドとプレキシン受容体による細胞間の通信は,組織ホメオスタシス,形態変生,神経発育,癌,免疫反応に不可欠です.
  • SEMA4DとSema6Aは脊椎動物のセマフォリンで,それぞれプレキシンB受容体とプレキシンA受容体を通して信号を発する.
  • セマフォリン-プレキシンの相互作用とプレキシンのシグナル伝達特異性の構造的基礎は,以前は知られていなかった.

研究 の 目的:

  • セマフォリン-プレキシンの相互作用とシグナル伝達の基礎となる構造的メカニズムを解明する.
  • 細胞外特異性とプレキシンシグナル伝達を制御するメカニズムを決定する.

主な方法:

  • X線結晶学を用いて,セマフォリン-プレキシン複合体と個々の構成要素の構造を決定した.
  • これらの相互作用の機能的結果を調査するために,生体物理学的および細胞学的測定法が使用されました.

主要な成果:

  • 結晶構造は,セマフォリンジマーが2つのプレキシン分子と結合し,信号伝送に不可欠な二価2:2複合体を形成する方法を明らかにしました.
  • プレキシンと結合するモノメリックセマフォリンでは,シグナリングが誘発されず,アビディティの重要性を強調しています.

さらに関連する動画

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
10:30

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Published on: January 12, 2014

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
11:56

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

Published on: June 16, 2017

関連する実験動画

Last Updated: May 13, 2026

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay
12:52

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay

Published on: March 4, 2011

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
10:30

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Published on: January 12, 2014

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
11:56

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

Published on: June 16, 2017

  • セマフォリン結合領域 (七片のβプロペラ) を含む保存された構造構造は,共通の相互作用モードを媒介し,変異は特異性を決定する.

    • 結論:

    • セマフォリン-プレキシンのシグナリングは,セマフォリン-安定したプレキシンの二分化によって誘発され,その後にクラスタリングが起こる可能性があります.
    • この発見は,セマフォリン-プレキシンの相互作用と,多様な生物学的プロセスにおけるそれらの役割を理解するための構造的枠組みを提供します.
    • 保存された構造要素と変数構造要素は,共通のシグナル伝達機構と,異なるセマフォリン-プレキシンペアで観察された特異性の両方を説明します.