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関連する概念動画

The Extracellular Matrix01:42

The Extracellular Matrix

In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...

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関連する実験動画

Updated: Jun 8, 2026

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

構造的に調整可能なDNAベースの細胞外マトリックスです.

Faisal A Aldaye1, William T Senapedis, Pamela A Silver

  • 1Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, United States. faisal_aldaye@hms.harvard.edu

Journal of the American Chemical Society
|October 8, 2010
PubMed
まとめ
この要約は機械生成です。

研究者らは,細胞の支架のための新しいDNA/タンパク質マトリクスを作成した. これらのプログラム可能な生体材料は,細胞の行動と構造を正確に制御し,研究アプリケーションのために自然細胞外環境を模倣します.

さらに関連する動画

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
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A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

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関連する実験動画

Last Updated: Jun 8, 2026

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
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A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

科学分野:

  • バイオマテリアル科学 バイオマテリアル科学
  • 分子工学は分子工学である.
  • 細胞生物学 細胞生物学

背景:

  • 細胞外マトリックス (ECM) は,細胞の行動と組織構造に不可欠です.
  • 現在のECMモデルは,しばしば正確な構造制御とプログラム性が欠けている.
  • DNAナノテクノロジーをタンパク質工学と統合することで,合成ECMの新たな可能性が生まれます.

研究 の 目的:

  • DNAナノテクノロジーとタンパク質工学を組み合わせて,人工細胞外マトリックスの新しいクラスを開発する.
  • これらのDNA/タンパク質マトリックスが,ex vivoの細胞構造に役立つことを実証する.
  • 自然な細胞ニッチを模倣するプログラム可能なECMを作成するためのモジュラープラットフォームを確立する.

主な方法:

  • タンパク質工学技術とDNAナノテクノロジーの原理を組み合わせた.
  • 製造されたDNA/タンパク質ベースのマトリックスで,細胞構造を施す.
  • 設計された単一鎖DNAドメインは,マトリックス持続長と硬さを調整します.
  • エンジニアリングされたマトリックスに培養されたヒト子宮頸がん細胞.
  • 細胞粘着,活力,移動,細胞骨格の配置,p-FAKシグナル伝達,およびFOXO1a転写因子の局所化を分析した.

主要な成果:

  • 人間の子宮頸がん細胞は,DNA/タンパク質マトリックスに強い粘着性,高い生存能力,そして急速な移動を示した.
  • DNA/タンパク質マトリクスの構造的調節性は,DNAドメインのエンジニアリングによって達成されました.
  • マトリックスの硬さと持続長さは,細胞の細胞骨格組織と形状に影響を与えました.
  • 細胞信号伝達 (p-FAK) と転写因子局所化 (FOXO1a) は,設計されたマトリックス特性によって調節された.

結論:

  • プログラム可能な,DNA/タンパク質ベースの人工細胞外マトリックスの新しいクラスが成功裏に開発されました.
  • これらのマトリクスは,ex vivo細胞構造の構築と細胞-マトリクスの相互作用の研究のための多用途のプラットフォームを提供します.
  • 簡単でモジュール化された構造は,自然細胞外ニッチの体系的な複製と調査を可能にします.