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Updated: Jun 8, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

熱い酸化剤である3-NO2Y122ラジカルは,リボヌクレオチド還元酵素におけるコンフォーマーションゲーティングを明らかにする

Kenichi Yokoyama1, Ulla Uhlin, JoAnne Stubbe

  • 1Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, United States.

Journal of the American Chemical Society
|October 9, 2010
PubMed
まとめ

この研究は,改変されたEscherichia coliのリボヌクレオチド還元酵素 (RNR) が,ニトロチロシル基 (NO(2) Y(•)) を使用して,陽子結合電子伝送 (PCET) を解離する方法を示しています. この解離は,酵素の速度を制限するコンフォメーションゲーティングステップを明らかにし,RNR触媒への新しい洞察を提供します.

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