Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Bioactivation and Tissue Toxicity01:25

Bioactivation and Tissue Toxicity

Bioactivation is a metabolic process that transforms less reactive substances into highly reactive metabolites, initiating tissue toxicity. This transformation can lead to various toxic effects, including carcinogenesis and teratogenesis. Reactive metabolites are classified into two main types: electrophiles and free radicals.Electrophiles are electron-deficient species and are produced primarily by the enzyme cytochrome P-450 during the metabolism of compounds containing carbon, nitrogen, or...
Cellular Injury I: Introduction01:00

Cellular Injury I: Introduction

Cellular injury occurs when a cell cannot maintain homeostasis or adapt to stressors such as hypoxia, toxins, or trauma. Depending on severity and duration, injury may be reversible, allowing recovery, or irreversible, leading to cell death.General Mechanisms of Cell InjuryAlthough causes vary, most cellular injuries arise from a few key mechanisms that disrupt essential functions and often amplify one another. Cell survival depends on the extent and balance of these disturbances.ATP depletion...
Radical Autoxidation01:20

Radical Autoxidation

The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
Cellular Injury IV: Necrosis01:16

Cellular Injury IV: Necrosis

Necrosis is a form of irreversible cell death caused by severe injury such as ischemia, toxins, or trauma. Unlike programmed cell death, it is an uncontrolled, pathological process that typically provokes inflammation in surrounding tissues.Pathophysiologic ChangesNecrosis begins when cells sustain critical damage, leading to swelling of organelles, particularly mitochondria, and rapid ATP depletion. As energy levels decline, membrane ion pumps fail, leading to calcium influx and eventually,...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

[Retracted] Mechanism of cell death induced by silica nanoparticles in hepatocyte cells is by apoptosis

International journal of molecular medicine·2026
Same author

Epigenetic consequences of DNA damage.

Molecular cell·2026
Same author

[Retracted] Silica nanoparticles induce cardiomyocyte apoptosis via the mitochondrial pathway in rats following intratracheal instillation.

International journal of molecular medicine·2026
Same author

Phase 2a/b randomised placebo-controlled dose-escalation trial of triheptanoin for ataxia-telangiectasia: treating mitochondrial dysfunction with anaplerosis.

EBioMedicine·2025
Same author

Beyond the β-α-β Fold: Characterization of a SnoaL Domain in the Tautomerase Superfamily.

Biochemistry·2025
Same author

Unraveling the nexus: Genomic instability and metabolism in cancer.

Cell reports·2025

関連する実験動画

Updated: Jun 7, 2026

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
10:57

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

Published on: October 13, 2019

酸化ストレスによるATM活性化.

Zhi Guo1, Sergei Kozlov, Martin F Lavin

  • 1Howard Hughes Medical Institute, Department of Molecular Genetics and Microbiology, and Institute for Cellular and Molecular Biology (ICMB), University of Texas at Austin, Austin, TX 78712, USA.

Science (New York, N.Y.)
|October 23, 2010
PubMed
まとめ

酸化は,DNA損傷とは関係なく,アタキア・テランジエクタジア変異 (ATM) タンパク質キナーゼを直接活性化します. この発見は,ATMが人間の細胞における酸化ストレスと反応性酸素種の重要なセンサーであることを明らかにしています.

科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • セルラー・シグナリング

背景:

  • アタクシア・テランジエクタシア変異 (ATM) タンパク質キナーゼは,DNA損傷反応の重要な調節体であり,主にMre11-Rad50-Nbs1 (MRN) 複合体によるDNA二重鎖断裂 (DSB) によって活性化されます.
  • ATM欠乏細胞は,酸化ストレスを含む様々な細胞攻撃に対して過敏感を示し,DSBの修復を超えた役割を示唆する.

研究 の 目的:

  • 酸化ストレス下でのATMの直接活性化メカニズムを調査する.
  • ATMがDNA二重鎖の断裂とMRN複合体とは無関係に活性化できるかどうかを判断する.

主な方法:

  • ATMの活性化と二酸化を検出するための生化学的測定法.
  • ATMにおけるクリティカルなシステイン残留物のサイト指向型変異.
  • 酸化剤への反応としてATMの活性化を評価するための細胞測定法.

主要な成果:

  • 酸化はATMの活性化を直接誘導し,DNADSBとMRN複合体から独立して,二硫化物とクロスリンクされたダイマーを形成します.
  • 特定のシステイン残留物の変異により,酸化経路経由でATMの活性化が取り消された.
  • この経路は,酸化ストレス条件下で観察されたATM活性化を説明します.

さらに関連する動画

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
10:24

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

Analysis of Oxidative Stress in Zebrafish Embryos
11:05

Analysis of Oxidative Stress in Zebrafish Embryos

Published on: July 7, 2014

関連する実験動画

Last Updated: Jun 7, 2026

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
10:57

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

Published on: October 13, 2019

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
10:24

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

Analysis of Oxidative Stress in Zebrafish Embryos
11:05

Analysis of Oxidative Stress in Zebrafish Embryos

Published on: July 7, 2014

結論:

  • ATMは,活性酸素種 (ROS) の直接センサーとして機能します.
  • オキシダティブ・モディフィケーションは,ATMの活性化のための新しい,直接的な経路を表しています.
  • この発見は,細胞のストレス反応におけるATMの役割の理解を広げています.