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関連する概念動画

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Updated: Jun 7, 2026

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
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Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

S-RNaseベースの自己不互換性における非自己認識の協力システム

Ken-ichi Kubo1, Tetsuyuki Entani, Akie Takara

  • 1Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan.

Science (New York, N.Y.)
|November 6, 2010
PubMed
まとめ
この要約は機械生成です。

花咲く植物は,遺伝子多様性を確保するために自己不適合性を利用します. ペチュニアでは,複数のS-ロカスF-ボックス (SLF) タンパク質が協力して,多様な非自己S-リボヌクレアスを認識し,互換性のある受粉を可能にします.

さらに関連する動画

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
08:08

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Published on: June 16, 2020

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

関連する実験動画

Last Updated: Jun 7, 2026

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
07:12

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
08:08

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Published on: June 16, 2020

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

科学分野:

  • 植物の生殖生物学 植物の生殖生物学
  • 分子遺伝学 分子遺伝学
  • 進化生物学の進化生物学について

背景:

  • 自己不適合性 (SI) は,近親交配を防止し,アウトクロッシングを通じて遺伝的多様性を促進する,開花植物における重要なメカニズムです.
  • Solanaceaeファミリーでは,S-ロカスF-ボックス (SLF) 遺伝子が花粉決定因子をコードし,S-リボ核酸酶 (S-RNases) は SI.でピスティル決定因子を表しています.
  • SI研究の重要な質問は,花粉決定因子 (SLF) が,SLFの低アルレル多様性を考慮して,幅広い非自己ピスティル決定因子 (S-RNases) を認識する方法です.

研究 の 目的:

  • SLFタンパク質がペチュニアの様々なS-RNaseを認識するメカニズムを調査する.
  • 非自己S-RNaseの認識における花粉決定因子の協力的な性質を明らかにする.
  • Solanaceae.における自己不適合性の分子基礎を理解する.

主な方法:

  • 生体内の機能分析は,花粉管内のSLFタンパク質の活性をテストするために使用されました.
  • SLFとS-RNaseタンパク質の結合特異性を決定するために,タンパク質相互作用アッセイが行われました.
  • SLFとS-RNaseアレルの比較配列解析が行われました.

主要な成果:

  • ペチュニアでは,少なくとも3種類の異なる異なるSLFタンパク質が,機能的な花粉の決定因子として特定されました.
  • 各種のSLFタンパク質は,非自己S-RNaseの特定のサブセットを認識する能力を示した.
  • これらの発見は,SLFとS-RNaseの1対1の相互作用ではなく,共同認識システムを示唆しています.

結論:

  • この研究は,ペチュニアの自己不互換性における複数のSLFタンパク質によって仲介された協同的な非自己認識システムを示しています.
  • この協働メカニズムは,非セルフS-RNaseの幅広いスペクトルを認識することを可能にし,SLFの認識能力に関する以前のパズルを解決します.
  • この発見は,植物の自己相容れない状態と遺伝的多様性の維持を支える分子機構のより深い理解に貢献します.