バクテリアのリボヌクレアゼPホロ酵素とtRNAの複合体の構造
Nicholas J Reiter1, Amy Osterman, Alfredo Torres-Larios
1Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, USA.
Nature
|November 16, 2010
まとめ
tRNAに結合するリボヌクレアゼP (RNase P) ホロ酵素の結晶構造は,その触媒機構を明らかにする. この研究では,RNA-RNAの相互作用とtRNA処理に不可欠な活性部位を明らかにしています.
科学分野:
- バイオケミストリー バイオケミストリー
- 構造生物学 構造生物学とは
- 分子生物学は分子生物学である.
背景:
- リボヌクレアースP (RNase P) は,tRNA5'-endの成熟を担う重要な酵素である.
- RNase Pはリボエンザイムであり,その触媒的活動は主にRNAベースのものである.
- RNase Pの構造を理解することは,その触媒機構の解読の鍵です.
研究 の 目的:
- テルモトガの海洋RNase Pホロ酵素の高解像度結晶構造をtRNAと複合して決定する.
- 基質の認識と触媒を制御する分子相互作用の解明.
- RNase P機能の普遍的なメカニズムについての洞察を提供するために.
主な方法:
- 154 kDa の RNase P-tRNA 複合体の構造を取得するために,X線結晶学が採用されました.
- プレ-tRNAと金属イオンによる浸漬実験を行い,基質結合部位と触媒部位を特定しました.
- 構造分析は,RNA-RNAの相互作用,タンパク質-RNAのインターフェース,および活性部位の組成に焦点を当てた.
主要な成果:
- 結晶構造は,形状の互補性,特定の接触,塩基のペアリングを通して複雑なRNA-RNA認識を明らかにします.
- タンパク質コファクターは,保存されたRNAモジュールと結合し,成熟したtRNAではなく,プレ-tRNAリーダー配列と相互作用します.
- 活性部位は,フォスファート骨幹元素,保存された尿素,および必須金属イオンで構成され,保存された触媒機構を示しています.
結論:
- この研究は,RNase P媒介のtRNA処理の詳細な構造的基礎を提供します.
- この発見は,RNAとタンパク質の両方の成分が基板結合と触媒の作用を強調しています.
- 保存された特徴は,RNase Pが異なる生物体全体で普遍的な触媒メカニズムを持っていることを示唆しています.
関連する概念動画
RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...


