C-ファクター:M. xanthusの果実体形態変異に必要な細胞間信号伝達タンパク質

S K Kim¹, D Kaiser

¹Department of Biochemistry, Stanford University School of Medicine, California 94305.

Cell

|April 6, 1990

まとめ

C因子タンパク質は,果実体の発達中に細胞集積と胞子分化に不可欠です. この形態遺伝的パラクリン信号は,csgA変異細胞の正常な発達を回復する.

科学分野:

細胞生物学と発達生物学
分子生物学は分子生物学である.

背景:

csgA遺伝子産物は,細胞集積,胞子分化,および果実体の発達中の遅い遺伝子発現に不可欠です.
csgAが欠けている変異細胞は,これらの発達過程で欠陥を示します.

研究の目的:

果実体の発達におけるcsgA遺伝子機能に起因する分子因子を特定し,特徴づけること.
細胞発達におけるこの因子の性質と活性を調べる.

主な方法:

17kdのポリペプチド (C因子) を新生野生型果実体から浄化する.
csgA変異細胞の正常な発達を回復するC因子活性に関する測定.
浄化されたC因子のアミノ酸配列解析.

主要な成果:

17kdのポリペプチド,C-ファクターと呼ばれるものが精製され,csgA遺伝子の産物として特定されました.
C-ファクターは,ナノモラー濃度 (1-2 nM) でcsgA変異細胞に正常な果実体の発達を回復させた.
C-ファクター活動は,発達中の細胞に特異的であり,成長中の細胞やcsgA変異体には存在しない.

結論:

C-ファクターは,ディクティオステリウム発育に不可欠な形態遺伝的パラクリン信号です.
csgA遺伝子製品は,重要な発達移行を調節するシグナル分子の役割を果たします.

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