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ウイルスRNAエレメントによって,トリプルヘリクスの組み立てを通じて,ポリー (Poly) Aの尾の認識
Rachel M Mitton-Fry1, Suzanne J DeGregorio, Jimin Wang
1Department of Molecular Biophysics and Biochemistry (MB&B), Howard Hughes Medical Institute (HHMI), Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536-9812, USA.
Science (New York, N.Y.)
|November 27, 2010
まとめ
カポシのサルコマ関連ヘルペスウイルスは,核RNA要素 (ENE) を使って,腐敗を防ぐ. このENEは, RNAクランプとして作用し,ポリ (A) 尾を保護するためにトリプルヘリックスを形成します.
科学分野:
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
- RNA 生物学 RNA 生物学
背景:
- カポシのサルコマ関連ヘルペスウイルスは,豊富な核ポリアデニル核 (PAN) RNAを産生する.
- PAN RNAには,発現と核保持要素 (ENE) が含まれており,その分解を防止します.
- ENEは,PAN RNAの多項A尾を保護するために,ボックスH/ACA小核核RNA (snoRNAs) を真似すると仮定された.
研究 の 目的:
- ENE媒介のPAN RNAの腐敗防止の構造的基礎を決定する.
- ENEがPANRNAのポリ (A) テイルと相互作用するメカニズムを解明する.
主な方法:
- X線結晶学を用いて,ENEの核の構造を決定し,オリゴ(A) ((9) RNA.と複合した.
- 観察されたRNA相互作用の機能的意義を評価するためにデデニレーションアッセイが行われました.
主要な成果:
- 結晶構造は,ENEが,スノRNAとは異なり,標的RNAとメジャー・グローブ・トリプルヘリックスを形成していることを明らかにした.
- A-マイナー相互作用が特定され,ENEとポリA尾の間の結合インターフェースを拡張しました.
- デデニレーションアッセイは,RNAの分解を防止するトリプルヘリクスの重要な役割を確認しました.
結論:
- ENEは,分子内RNAクランプとして機能し,PANRNAの多分子A尾を隔離します.
- このシーケストレーションメカニズムは,RNAの分解の開始を防止し,PAN RNAの安定性を確保します.
- この発見は,ウイルスのノンコーディングRNAsによるRNA分解の調節に関する新しい洞察を提供します.
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