遺伝子型特異的な変形剤によるトランスゲン発現とインプリントの表遺伝子制御
N D Allen1, M L Norris, M A Surani
1Department of Molecular Embryology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, England.
Cell
|June 1, 1990
まとめ
遺伝子型特異的変異遺伝子は,トランスゲン発現とDNAメチル化を制御する. TKZ751ロカスにおける表遺伝的変異は,マウスでは不可逆的になり,遺伝子浸透とインプリントに影響を及ぼします.
科学分野:
- 遺伝学 遺伝学とは
- エピジェネティクス エピジェネティクス
- 分子生物学は分子生物学である.
背景:
- トランスゲン発現とDNAメチル化の調節は,遺伝子制御を理解するために極めて重要です.
- 遺伝的背景は,表遺伝的改変と遺伝子発現パターンに影響を与える可能性があります.
研究 の 目的:
- TKZ751トランスゲンロクスの発現とDNAメチル化を制御する遺伝子型特異変異遺伝子の役割を調査する.
- 異なった遺伝的背景 (DBA/2,129,BALB/c) がトランスゲンの表遺伝的変異に与える影響を決定する.
- 遺伝パターンと,世代を超えた表遺伝的変化の逆転性を調べる.
主な方法:
- 異なるマウスの遺伝子背景におけるトランスゲンロカスTKZ751発現とDNAメチル化の分析.
- BALB/c遺伝子背景の母性遺伝効果を調査する.
- 次世代にわたる表遺伝的変化を追跡する.
主要な成果:
- DBA/2と129の遺伝子背景がTKZ751の発現を高めました.
- BALB/c 背景抑制表現,特に母親からの遺伝.
- エピジェネティック変異は累積的であり,BALB/cマウスでは3回の生殖経路を経て不可逆的となった.
- ゲルムラインは,TKZ751の位置で以前に獲得された表遺伝的変化を逆転させることができませんでした.
結論:
- 遺伝子型特異的な変異遺伝子は,TKZ751ロカス.の浸透性と発現性を調節する.
- DNAメチレーションを含む表遺伝的変異は,この調節において重要な役割を果たします.
- この研究は,特定の遺伝的文脈におけるTKZ751の場所での生殖線表遺伝的再プログラミングの失敗を強調し,親のインプリントに影響を与えています.
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