メソデーム誘導を司るXenopus laevisの細胞系から得られたアクティウシンのような因子
A J van den Eijnden-Van Raaij1, E J van Zoelent, K van Nimmen
1Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Nature
|June 21, 1990
まとめ
Xenopus laevis ectoderm explantsは,成長因子を用いてメソデームを形成するように誘導することができる. 研究者らは,XTC細胞からのXenopus laevisメソダーム誘発因子 (MIF) が哺乳類のアクティビンの同型であることを確認した.
科学分野:
- 発達生物学 発達生物学とは
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- 両生類の胚形成におけるメソデーム誘導は,発達にとって極めて重要です.
- 変形成長因子β (TGF-β) のような成長因子は,このプロセスを in vitro で真似することができます.
- Xenopus laevisのXTC細胞からの特定のメソデルマ誘発因子 (MIF) は,TGF-βのような性質を示しているにもかかわらず,未確認のままです.
研究 の 目的:
- Xenopus laevis XTC細胞によって生成されるメソダーマ誘発因子 (MIF) の性質を調査する.
- XTC細胞クローンにおけるMIF活性とアクティビンの活性との関係を分析する.
- XTC-MIFが哺乳類のアクティビンのXenopus同類であるかどうかを判断する.
主な方法:
- 異質なXTC細胞集団から均質な細胞系を分離する.
- 異なるXTCクローンにおけるメソダーマ誘導因子 (MIF) 産生を測定する.
- 変形成長因子β (TGF-β) の活性と細胞抽出物におけるアクティビンのような活性を測定する.
- MIFの活性とXTC細胞系におけるアクティビンの活性を比較する.
主要な成果:
- 1つのXTCクローンであるXTC-GTX-11のみが,MIFの重要な活動を生み出した.
- このクローンは,他のクローンと比較して,TGF-β活性を含む,既知の成長因子の同様の生産を示しました.
- XTC細胞およびそのクローンにおけるMIF活性とアクティビンの活性との間の明確な一貫した相関が観察されました.
- アクティビンAは,強力なメソダーマ誘発因子として特定されました.
結論:
- Xenopus laevis XTC細胞からのメソダーマ誘発因子 (MIF) は,アクティビンの活動と強く関連しています.
- XTC-MIFは,哺乳類のアクティビンのXenopus同型として特定されています.
- この発見は,Xenopus laevisの胚生成におけるメソデルマ誘導の分子基盤を明らかにしています.
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