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Updated: Jun 6, 2026

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
13:59

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Published on: December 12, 2013

高分子量タンパク質の分子構造とダイナミクスのNMRプローブとしてアラニンメチル群

Raquel Godoy-Ruiz1, Chenyun Guo, Vitali Tugarinov

  • 1Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.

Journal of the American Chemical Society
|December 9, 2010
PubMed
まとめ

アラニンメチル群は,タンパク質の構造とダイナミクスを研究するための貴重なNMRプローブです. 選択的ラベリングは,これらのメチル基が高度に順序付けられていることを明らかにし,マラート合成酵素Gのような大きなタンパク質のアプリケーションを強化します.

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NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is broad and...
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Proton (¹H) NMR: Chemical Shift

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Absorption signals of all the protium nuclei in a...
Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic aliphatic amines show...
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¹H NMR of Labile Protons: Temporal Resolution

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Mass Spectrometry: Amine Fragmentation

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