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Updated: Jun 6, 2026

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

酵素活性部位におけるX線およびNMR結晶学:トリプトファン合成酵素におけるインドリンキノノイド中間体

Jinfeng Lai1, Dimitri Niks, Yachong Wang

  • 1Department of Chemistry, University of California, Riverside, California 92521, USA.

Journal of the American Chemical Society
|December 15, 2010
PubMed
まとめ

固体NMRとX線結晶学を組み合わせると,トリプトファン合成酵素の重要な中間物質の詳細な構造が明らかになる. このアプローチは,触媒処理中のプロトネーション状態を定義することによって,酵素機構を明確にします.

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