関連する実験動画

Updated: Jun 6, 2026

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
07:19

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

Published on: February 1, 2011

DNA分子におけるメチルサイトシンとヒドロキシメチルサイトシンの区別

Meni Wanunu1, Devora Cohen-Karni, Robert R Johnson

  • 1Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. wanunu@sas.upenn.edu

Journal of the American Chemical Society
|December 16, 2010
PubMed
まとめ

この研究では,5-メチルサイトシン (mC) と,5-ヒドロキシメチルサイトシン (hmC) のDNA改変を区別するための物理的方法が紹介されています. 固体ナノポールは,これらの表遺伝子マーカーを素早く分化することができ,病気の研究に役立ちます.

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Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
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Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
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Mismatch Repair

Overview
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Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...