関連する実験動画
Updated: Jun 16, 2026
09:49
Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
Published on: August 14, 2010
免疫グロブリン重鎖トランスゲンの同型スイッチングは,異なる染色体間のDNA再結合によって起こります
R M Gerstein1, W N Frankel, C L Hsieh
1Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Cell
|November 2, 1990
まとめ
トランスジェニックマウスは,抗体遺伝子のスイッチングが染色体間の間で起こりうることを示しています. この染色体間再結合は,通常のスイッチングと同じメカニズムを使用し,B細胞腫瘍の転位を引き起こす可能性があります.
科学分野:
- 免疫学 免疫学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- 免疫グロブリン・ム重鎖トランスゲンを持つトランス遺伝子マウスは,同型変異を受けます.
- このトランスゲンスイッチングのメカニズムは,以前は特徴づけられていなかった.
研究 の 目的:
- 免疫グロブリンの重鎖トランスゲン同型変異のメカニズムを特徴づける.
- 異なった染色体間で同型変異が起こるかどうかを判断する.
主な方法:
- バッククロスマウスの遺伝子マーカーとして多形内生レトロウイルスを用いて,トランスゲンの挿入部位の局所化.
- イソタイプスイッチング中のDNA再結合の分析.
主要な成果:
- muトランスゲンの挿入部位は,染色体5にマッピングされました.
- 固有の免疫グロブリンの重鎖ロクスは,染色体12にある.
- トランスゲン同型スイッチングは,染色体間DNA再結合を含むことが示されました.
- 同じ酵素メカニズムは,正常および染色体間スイッチングの両方を媒介しているようです.
結論:
- 抗体遺伝子同型スイッチングは,染色体間で起こり得る.
- 同型変異のメカニズムは,c-myc遺伝子を含むB細胞腫瘍で観察されたものと類似した染色体転位を引き起こす可能性があります.
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