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関連する概念動画

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response01:15

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...

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関連する実験動画

Updated: Jun 5, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

クリプトクローム1によるフィードバック抑制の遅延は,日経時時計機能のために必要です.

Maki Ukai-Tadenuma1, Rikuhiro G Yamada, Haiyan Xu

  • 1Laboratory for Systems Biology, RIKEN Center for Developmental Biology, Chuo-ku, Kobe, Hyogo, Japan.

Cell
|January 18, 2011
PubMed
まとめ

クリプトクローム1 (Cry1) の発現を遅らせることは,哺乳類の昼夜時計が正しく機能するために不可欠です. この研究は,特定のDNA要素がCry1を制御する方法を示しています.

さらに関連する動画

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
07:44

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

関連する実験動画

Last Updated: Jun 5, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
07:44

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

科学分野:

  • クロノバイオロジーはクロノバイオロジーを用います.
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • 哺乳類の昼夜時計は,日々の生物学的リズムを調節する.
  • フィードバック抑制は時計の機能に不可欠ですが,直接的な証拠は不足しています.
  • クリプトクローム1 (Cry1) は,昼夜リズム調節に関与する重要なタンパク質です.

研究 の 目的:

  • 哺乳類の昼間時計における遅延フィードバック抑制の必要性を調査する.
  • クリプトクローム1 (Cry1) の夕方発現の基礎となる規制メカニズムを解明する.
  • シルカディアンリズム維持におけるCry1発現タイミングの役割を決定する.

主な方法:

  • DボックスとRREを含むCry1遺伝子規制要素の分析.
  • Cry1.1のための合成複合材料プロモーターの製造と試験.
  • 段階ベクトルモデルの開発と応用.
  • Cry1(-/-):Cry2(-/-) 細胞における遺伝的補完分析.

主要な成果:

  • 昼間 (Dボックス) と夜間 (RREs) の要素の組み合わせが,夜間Cry1表現を駆動する.
  • 合成プロモーターはこの表現パターンを再現した.
  • これらの要素を調整することによって,相遅延の調節が観察されました.
  • ノックアウト細胞の昼夜リズムを回復するために,Cry1発現を大幅に遅らせることが必要でした.
  • 長期のCry1遅延により,昼夜振動が遅くなりました.

結論:

  • クリプトクローム1 (Cry1) 転写の相遅延は,哺乳類の時計機能の重要な要件です.
  • プロモーターとエンハンサーの要素の相互作用が,Cry1の発現タイミングを決定する.
  • Cry1の発現の正確なタイミングは,昼間の振動の速度に影響する.