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関連する概念動画

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

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関連する実験動画

Updated: Jun 4, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

化学的に改変されたオリゴヌクレオチドによって誘発される結合誘導性RNA干渉.

Deepak Kumar1, Sang Hoon Kim, Yohei Yokobayashi

  • 1Department of Biomedical Engineering, University of California, Davis, Davis, California 95616, USA.

Journal of the American Chemical Society
|February 8, 2011
PubMed
まとめ

この研究は,精密な遺伝子サイレンシングのための新しい化学的に誘導可能なRNA干渉 (RNAi) プラットフォームを導入しています. このシステムは,複数の遺伝子の組み合わせ制御を可能にし,遺伝子機能の研究と薬剤発見を加速します.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝子規制 遺伝子規制
  • バイオテクノロジー バイオテクノロジー

背景:

  • 化学的に誘導可能なRNA干渉 (RNAi) は,様々な用途の遺伝子発現に対する正確な制御を提供します.
  • 既存のRNAi方法では,複雑な遺伝子相互作用の研究に必要な組み合わせ制御が欠けている可能性があります.

研究 の 目的:

  • 組み合わせ遺伝子サイレンシングのための新しい誘導性RNAiプラットフォームを開発する.
  • 化学誘発剤を用いた遺伝子発現の時間的・空間的制御を可能にする.

主な方法:

  • オリゴヌクレオチドセンサステムループとRNAiエフェクタドメインを備えたモジュラーRNAアーキテクチャを設計した.
  • 化学的に改変されたオリゴヌクレオチドを誘導体として利用し,RNAの構造変化を誘発した.
  • 構造的なシフト時に遺伝子サイレンスを行うためのレバレッジされたRNAi機械.

主要な成果:

  • 新しい誘導性RNAiプラットフォームを成功裏に実証しました.
  • 化学的に誘発されたRNAの構造的変化を通じて遺伝子サイレンシングを誘発する能力を示した.
  • 2つの遺伝子の組み合わせ調節のためのシステムを確立しました.

さらに関連する動画

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

関連する実験動画

Last Updated: Jun 4, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

結論:

  • 開発されたプラットフォームは,複雑な遺伝子相互作用を研究するための強力なツールを提供します.
  • このアプローチは,潜在的な薬物標的のスクリーニングを加速します.
  • 遺伝子治療と機能的ゲノミクス研究における潜在的な応用を提供します.