lncRNAsは,STAU1媒介のmRNA分解を,Alu要素経由で3' UTRsとの二重複製によってトランザクティブにします
Chenguang Gong1, Lynne E Maquat
1Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA.
Nature
|February 11, 2011
まとめ
長い非コーディングRNA (lncRNAs) は,Staufen 1 (STAU1) に結合することによってmRNAの分解を媒介することができます. Alu元素によって促進されるこの相互作用は,遺伝子調節とRNA崩壊経路のための新しいメカニズムを明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- RNA 生物学 RNA 生物学
- 遺伝子規制 遺伝子規制
背景:
- Staufen 1 (STAU1) 媒介のメッセンジャーRNA分解 (SMD) は,トランスレーション活性mRNAを標的とする.
- ARF1 mRNAの以前に特定されたSTAU1結合部位には,特定の構造的特徴がありました.
- 比較可能な構造は,他のSMDのターゲットでは見つかりませんでしたが,理解のギャップを示しています.
研究 の 目的:
- STAU1が他のmRNA標的と結合するメカニズムを解明する.
- SMDにおけるノンコーディングRNAとアルウ元素の役割を調査する.
- 遺伝子調節における lncRNA の新しい機能を特定する.
主な方法:
- mRNA 3' UTRsにおけるSTAU1結合部位の分析.
- mRNAとlncRNAにおけるAlu元素の相互作用を調査する.
- mRNAの衰退率に対するlncRNAの影響を評価する.
主要な成果:
- STAU1結合部位は,mRNA 3' UTRsとlncRNAsのAlu元素の間の塩基配列によって形成される可能性があります.
- 一つのlncRNAは,複数のSMD標的を調節することができます.
- 異なるlncRNAは,同じmRNAをターゲットにすることができます.
- Alu要素を持つすべてのmRNAが,SMDのターゲットになるわけではありません.
結論:
- lncRNAは,STAU1を特定のmRNAに勧誘することによって,トランス作用RNAエフェクターとして機能することができます.
- この相互作用は,mRNAの衰退を媒介し, lncRNAsとAlu元素の機能は以前は評価されていませんでした.
- これらのlncRNAは半STAU1結合部位RNA (1/2-sbsRNA) と呼ばれる.
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