Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Integrated single-cell and spatial transcriptomic profiling in ALS uncovers peripheral-to-central immune infiltration and reprogramming.

Nature neuroscience·2026
Same author

Design of Neuronal Supramolecular Scaffolds Integrating Cell Signaling and Electrical Conductivity.

ACS biomaterials science & engineering·2026
Same author

Peptide-Induced Ferroelectricity in Charge-Transfer Supramolecular Materials.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

A role for the cholinergic neuron circadian clock in RNA metabolism and mediating neurodegeneration.

Life science alliance·2025
Same author

TDP-43 dysfunction compromises UPF1-dependent mRNA metabolism in ALS.

Neuron·2025
Same author

Human iPSCs-based modeling unveils SETBP1 as a driver of chromatin rewiring in GATA2 deficiency.

Nature communications·2025
Same journal

Daily briefing: How cooperation built the world.

Nature·2026
Same journal

Deep-sea oddities and boatloads of other new species - June's best science images.

Nature·2026
Same journal

From cloning to gene-editing: the enduring legacy of Dolly the sheep.

Nature·2026
Same journal

Time to give hydration breaks the red card? What science says about keeping cool.

Nature·2026
Same journal

Universities are relying on AI-detection software to catch cheating. How well do the programs work?

Nature·2026
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: Jun 4, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

人間の誘発性多能幹細胞における体的コーディング変異.

Athurva Gore1, Zhe Li, Ho-Lim Fung

  • 1Department of Bioengineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.

Nature
|March 4, 2011
PubMed
まとめ
この要約は機械生成です。

誘発性多能幹細胞 (hiPS) は,再プログラム中に遺伝的変異を取得することができます. これらの得られた遺伝的変異は,表遺伝的変化とともに,安全な臨床応用のために遺伝的スクリーニングを必要とします.

さらに関連する動画

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
08:36

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Published on: August 20, 2015

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
08:25

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

Published on: April 3, 2012

関連する実験動画

Last Updated: Jun 4, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
08:36

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Published on: August 20, 2015

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
08:25

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

Published on: April 3, 2012

科学分野:

  • 細胞生物学 細胞生物学
  • 遺伝学 遺伝学とは
  • 幹細胞の研究について

背景:

  • 定義された転写因子は,成人哺乳類の細胞をエピジェネティックに再プログラムして誘発性多能幹細胞 (hiPS) にすることができます.
  • 再プログラミング後の単一ヌクレオチドレベルでのゲノム整合性は,主に特徴づけられていないままです.

研究 の 目的:

  • 誘発性多能幹細胞 (hiPS) が再プログラミング過程で遺伝子改変を受けるか否かを調査する.
  • 異なる方法によって生成されたhiPS細胞系における点変異の性質と頻度を評価する.

主な方法:

  • 5つの異なる再プログラム方法を使用して生成された22人のヒト誘発多能幹細胞 (hiPS) 細胞の配列決定.
  • 非同義,無意味,およびスプライス変異を含む点変異のためのタンパク質コーディング領域の分析.
  • hiPS細胞の突然変異と,その元の線維芽細胞の祖先細胞の比較.

主要な成果:

  • hiPSの細胞系では,エクソームあたり平均5つのタンパク質をコードする点変異が特定されました.
  • 癌に関連する遺伝子に変異が濃縮された.
  • 観察された突然変異の約半分は前身細胞に既に存在しており,残りの部分は再プログラム中に発生した.

結論:

  • 人間の誘発性多能幹細胞 (hiPS) は,表遺伝的再プログラムに加えて,遺伝的変異も獲得する.
  • これらの遺伝的変異には,がんに関連する遺伝子の潜在的に有害な変異が含まれます.
  • 臨床使用前にhiPS細胞の安全性を確保するために,包括的な遺伝子スクリーニングが不可欠です.