Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Dynamics of TFIIH and Spt4/5 during the transition from transcription initiation to elongation.

bioRxiv : the preprint server for biology·2026
Same author

mRNA COVID-19 vaccines: science versus misinformation.

RNA (New York, N.Y.)·2026
Same author

A Yeast-Based High-Throughput Screening Platform for the Discovery of Novel pre-mRNA Splicing Modulators.

ACS chemical biology·2026
Same author

TDP-43 controls RNA structure through high affinity lattice interactions.

bioRxiv : the preprint server for biology·2025
Same author

A Yeast-Based High-Throughput Screening Platform for the Discovery of Novel pre-mRNA Splicing Modulators.

bioRxiv : the preprint server for biology·2025
Same author

Lipid-Mediated Sequential Recruitment of Proteins Via Dual SLIPT and Dual SLIPT<sup>NVOC</sup> in Live Cells.

Bio-protocol·2025
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
関連記事をすべて見る

関連する実験動画

Updated: Jun 3, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
07:35

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

単一のスライセオソームの秩序的かつダイナミックなアセンブリ.

Aaron A Hoskins1, Larry J Friedman, Sarah S Gallagher

  • 1Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA.

Science (New York, N.Y.)
|March 12, 2011
PubMed
まとめ
この要約は機械生成です。

研究者は,スプライセソームの組み立てをリアルタイムで追跡し,プレ-mRNAからイントロンを除去するための連続的かつ可逆的なプロセスを明らかにしました. このオーダーされた経路は,代替スプライシングの規制に影響を与えます.

さらに関連する動画

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

関連する実験動画

Last Updated: Jun 3, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
07:35

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • バイオケミストリー バイオケミストリー

背景:

  • スプライセソームは,遺伝子発現のための重要な分子機構であり,前伝達 RNA (前mRNA) からイントロンの除去を触媒化する.
  • スプライソーム組立のダイナミクスを理解することは,遺伝子調節と代替スプライシングの基礎となるメカニズムを明らかにする鍵です.

研究 の 目的:

  • 単一のスプライソームのリアルタイムアセンブリを視覚化および分析する.
  • プレ-mRNA.spliceosomalサブコンプレックスとの関連性の順序とダイナミクスを決定する.
  • スプライシング経路へのプレ-mRNAのコミットメントポイントを調査する.

主な方法:

  • 精密な実験制御のために酵母遺伝子工学を用いた.
  • 分子相互作用を調査するために化学生物学ツールを使用しました.
  • 多波長光顕微鏡を用いて,全細胞抽出物における単分子可視化をリアルタイムで実現した.

主要な成果:

  • spliceosomalサブコンプレックスが,順序のある経路を通って,序列的にpre-mRNAに組み合わされることが示されました.
  • それぞれのサブコンプレックスとの関連が逆行可能なステップであることを発見しました.
  • pre-mRNAのスプライシングへのコミットメントは,初期のイベントではなく,アセンブリが進むにつれて漸進的に増加することを確立しました.

結論:

  • スプライソーム組立は,動的,秩序ある,および可逆的なプロセスです.
  • 段階的な組み立ては,代替スプライシングの規制に影響を与えます.
  • 開発された実験的アプローチは,近親細胞環境で他の複雑な分子機械を研究するための強力なツールを提供します.