MPFのサイクリンB2成分は,c-mos(xe) プロトオンコゲン産物の基板である
1Department of Pharmacology, University of Colorado School of Medicine, Denver 80262.
Cell
|June 1, 1990
まとめ
モス・プロト・オンコゲンは,成熟促進因子 (MPF) の重要な成分であるサイクリンB2を直接リン酸化する. この発見は,卵細胞の成熟中にMPF活性化のための新しいメカニズムを明らかにし,シクリンB2のモス媒介型リン酸化を伴う.
科学分野:
- 細胞および分子生物学
- 発達生物学 発達生物学について
- 腫瘍遺伝子 (オンコゲネス) とは
背景:
- 成熟促進因子 (MPF) は卵細胞の成熟に不可欠です.
- MPFには,cdc2キナーゼと複合したサイクリンB2が含まれています.
- MPFの活性化には,c-mosプロトオンコゲンが必要です.
研究 の 目的:
- MPFの活性化におけるc-mosプロトオンコゲンの役割を調査する.
- モスがサイクリンB2を直接リン酸化するかどうかを判断する.
- モス媒介によるMPF活性化のメカニズムを解明する.
主な方法:
- インビトロリン酸化アッセイでは,免疫プレシピテートされたv-mosとc-mosを用いた.
- リン酸化パターンを比較するためのフォスフォペプチド分析.
- アンチセンセスのオリゴヌクレオチド注射は,卵細胞内のc-mos (((xe) を消去する.
主要な成果:
- ウイルスのモス (v-mos) とクセノプスのc-mos (c-mos(xe)) の両方が,シクリンB2をインビトロで直接リン酸化する.
- mosによるリン酸化パターンは,cdc2キナーゼと似ています.
- c-mosのアブレーションによって,オオサイト抽出物におけるサイクリンB2のリン酸化が40%減少した.
結論:
- c-mosプロトオンコゲンは,サイクリンB2を直接リン酸化する.
- サイクリンB2のモス媒介型リン酸化は,卵細胞成熟期におけるMPF活性化における重要なステップである.
- この研究は,モスによってサイクリン成分を直接リン酸化するMPF活性化のための新しいメカニズムを明らかにしています.
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