キネトプラスティドの広範囲に編集されたミトコンドリアのトランスクリプトは,ATPaseサブユニット6に同類するタンパク質をコードする
G J Bhat1, D J Koslowsky, J E Feagin
1Seattle Biomedical Research Institute, Washington 98109-1651.
Cell
|June 1, 1990
まとめ
トライパノソーマ・ブルセイのミトコンドリアのMURF4遺伝子は,尿素の添加/消去によって広範なRNA編集を受けます. このプロセスは,MURF4がATPaseサブユニット6をコードするMURF4を明らかにする,機能的なオープン読書フレームを作成します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- トライパノソーマブルセイのようなキネトプラスティドのミトコンドリア遺伝子は,独特の特徴を示しています.
- MURF4遺伝子は,T. bruceiミトコンドリアに転写されていることが知られている.
研究 の 目的:
- T. brucei. のMURF4トランスクリプトの性質を調査する.
- MURF4遺伝子製品の機能を決定するために.
主な方法:
- T. brucei.からのMURF4トランスクリプト配列の分析.
- 予測されたアミノ酸配列と水道性プロフィールのバイオ情報分析.
- 既知のATPaseサブユニット6遺伝子との比較.
主要な成果:
- T. bruceiのMURF4トランスクリプトは,大規模なG/C鎖バイアスと異質なサイズを示しており,これは広範なRNA編集の兆候です.
- MURF4トランスクリプトにおける広範な尿素添加と消去は,開始/終了コドンと連続した開いた読み取りフレームを作成します.
- 予測されたMURF4アミノ酸配列と,様々な生物からのミトコンドリアのATPアゼサブユニット6との間のホモロジーが見つかりました.
- ミニサークルで潜在的ガイドRNA配列が特定されました.
結論:
- T. brucei の MURF4 遺伝子は,ミトコンドリアの ATPase サブユニット 6. をコードする.
- RNA編集は,T. bruceiミトコンドリアの機能的なATPアゼサブユニット6を生成するために不可欠です.
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