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preQ1リボスイッチの溶液と結晶構造を比較すると,カルシウムによって誘発された形状と動態の変化が明らかになる
Qi Zhang1, Mijeong Kang, Robert D Peterson
1Department of Chemistry and Biochemistry , University of California, Los Angeles, California 90095-1569, USA.
Journal of the American Chemical Society
|March 18, 2011
まとめ
バチルス・サブティリスのリボスイッチ・アプタメルは,そのリガンド結合状態において固有の柔軟性を表している. 結晶構造に存在するカルシウムイオンは,溶液中のその機能には不可欠ではありません.
科学分野:
- 分子生物学は分子生物学である.
- 構造生物学 構造生物学とは
- バイオケミストリー バイオケミストリー
背景:
- リボスイッチは,メタボライト結合を通じて遺伝子発現を調節する遺伝的要素です.
- リボスイッチのアプタマー領域は特定の代謝産物を結合し,形状の変化を誘発する.
- バシルス・サブティリスの溶液と結晶構造の間の不一致は存在します preQ(1) riboswitch aptamer.
研究 の 目的:
- バシルス・サブティリスの構造と動態を特徴付けるには,溶液NMRを用いてリボスイッチアプタマーをリガンド結合状態にします.
- アプタマーの構造と機能におけるカルシウムイオンの役割を調査する.
- 報告された溶液と結晶構造の違いを調和させるため.
主な方法:
- 溶液核磁共振 (NMR) スペクトロスコーピー. 溶液核磁共振 (NMR) スペクトロスコーピー. 溶液核磁共振 (NMR) スペクトロスコーピー. 溶液核磁共振 (NMR) スペクトロスコーピー. 溶液核磁共振 (NMR) スペクトロスコーピー. 溶液核磁共振 (NMR) スペクトロスコーピー. 溶液核磁共振 (NMR) スペクトロスコーピー.
- アプタマー-プレQ(1) 複合体の構造と動的特徴.
- カルシウムイオンに対するリガンド結合親和度測定.
主要な成果:
- アプタマー-プレQ(1) 複合体は溶液に固有の柔軟性があり,異なる領域で明確な動きをします.
- 特定のカルシウムイオンが,ミクロモラー親和性を持つリガンド誘発ポケットに結合する.
- カルシウムイオンは形状の変化を誘発しますが,溶液中のリガンド結合または折り畳みに必要ありません.
結論:
- バチルス・サブティリスのリボスイッチ・アプタメルは,そのリガンドに結合しても,溶液中の重要な形状の柔軟性を示す.
- 結晶と溶液の構造の間の観察された差異は,カルシウムイオン相互作用に起因する.
- カルシウムイオンは,溶液中のこのリボスイッチアプタマーの基本的な機能に不可欠ではありません.
関連する概念動画
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
01:23Transcriptional Regulation: Riboswitches
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
01:23Types of RNA
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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:44Termination of Translation
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
02:24Ribosome Profiling
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...