鳥類サルコマウイルスの変異遺伝子が,フォスフォタンパク質に関連したタンパク質キナーゼをコードする証拠
Cell
|October 1, 1978
まとめ
鳥肉腫ウイルス (ASV) 腫瘍遺伝子 src は6万ダルトンのフォスフォタンパク質をコードする, pp60. このタンパク質はキナーゼ活性を示しており,そのリン酸化がASV誘発の細胞変容に不可欠であることを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
- 腫瘍学 腫瘍学
背景:
- 鳥肉腫ウイルス (ASV) は,腫瘍を引き起こし,その src 遺伝子を介して細胞を変容します.
- 6万ダルトンのタンパク質は,SRCによって潜在的にコードされ,腫瘍の変容に関与しています.
研究 の 目的:
- 推定 src 遺伝子産物を分離し,特徴づけること.
- 6万ダルトンのタンパク質の機能と遺伝的起源を調査する.
主な方法:
- ASV腫瘍を患ったウサギの抗菌剤を用いた免疫プレシピテーション.
- 孤立したタンパク質の生化学的および遺伝的分析.
- インビトロリン酸化測定と温度感受性変異体分析.
主要な成果:
- 6万ダルトンのフォスフォタンパク質,pp60が特定され,srcによって暗号化されていることが確認されました.
- pp60には固有のタンパク質キナーゼ活性があり,免疫グロブリン重鎖をリン酸化します.
- pp60のリン酸化は温度に敏感であり,SRC機能と相関していた.
結論:
- pp60とその関連するタンパク質キナーゼ活性がASV src遺伝子によってコードされています.
- pp60のリン酸化は,腫瘍変異におけるその役割に不可欠である可能性が高い.
- pp60のリン酸化を司るキナーゼは,srcでコードされたキナーゼとは異なる可能性があります.
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