水のダイナミックな核極化による溶融球の非極性核における場所特有の水分化ダイナミクス
Brandon D Armstrong1, Jennifer Choi, Carlos López
1Department of Physics, University of California-Santa Barbara, Santa Barbara, California 93106, United States.
Journal of the American Chemical Society
|March 30, 2011
まとめ
アポミオグロービンの非極性核である.
科学分野:
- バイオフィジックス 生物物理学
- タンパク質のダイナミクス
- 構造生物学 構造生物学とは
背景:
- タンパク質の折りたたみには,非極性核から水の挤出が含まれます.
- 崩壊したタンパク質の中間状態の水分化は議論の余地があります.
- サイト固有のタンパク質水分化ダイナミクスを測定することは困難です.
研究 の 目的:
- サイト固有の解像度でタンパク質の水分化ダイナミクスを調査する.
- 異なるアポミオグロビン状態 (溶けた球体,ネイティブ,展開) の水のダイナミクスを探求する.
- タンパク質の折りたたみの中間物質における水の役割を調査する.
主な方法:
- オーバーハウザーのダイナミックな核極化 (ODNP) は,サイト特有の窒素酸化物スピンラベルを使用して0.35Tで.
- 電子パラマグネット共振 (EPR) による局所的極性および移動性に関する洞察.
- アポミオグロビンのスピンラベルから ~10 Å 以内の水のダイナミクスを探求する.
主要な成果:
- アポミオグロビン溶融球の非極性核は水分化されている.
- 溶けた球体内の水素化水は,重要な変換動態を示します (散発水よりも4〜6倍遅い).
- ネイティブアポミオグロビンは異質な水分化ダイナミクスを示し,展開状態は迅速に拡散する水を持っています.
結論:
- サイト固有のODNPは,タンパク質の水分化ダイナミクスを研究するための強力なツールです.
- タンパク質の水分化ダイナミックは折り畳みに依存し,異なるタンパク質状態によって異なる.
- 溶けた球体の内部タンパク質水は動的であり,以前の仮定に異議を唱える.
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