関連する実験動画
Updated: Aug 18, 2026

10:34
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
Published on: April 23, 2017
CD45ペプチドとの異常な形式の脂質結合
1Department of Pathology, Roger Williams General Hospital, Brown University, Providence, RI 02908.
まとめ
タンパク質キナーゼとフォスファタゼはミリストイラ化することができる. 血球形成性チロシンフォスファタゼであるグリコタンパク質CD45は,パルミテートではなく,異常な脂質結合を通じてミリスタートを組み込むことが判明しました.
科学分野:
- バイオケミストリー バイオケミストリー
- 細胞生物学 細胞生物学
- 免疫学 免疫学とは
背景:
- タンパク質ミリストイレーションは,タンパク質の局所化と機能に影響を与える可能性がある翻訳後の修正である.
- グリコタンパク質CD45は,免疫細胞のシグナル伝達に関与する重要な血液生成チロシンフォスファタゼです.
研究 の 目的:
- グリコプロテインCD45.5の脂肪酸アシレーションを調査する.
- CD45.5への脂質結合の性質を特徴づけるために
主な方法:
- CD45は代謝的に [3H]ミリスタートと [3H]パルミタートで標識されていました.
- 組み込みラベルを分析するためにペプチドマッピングとグリコシダース処理を使用しました.
- 軽度のアルカリメタノリシスに対する耐性を評価した.
主要な成果:
- CD45は [3H]ミリスタートを含有しているが, [3H]パルミタートを含有していない.
- ミリスタートラベルは,アミノ酸やサッカライドに代謝されませんでした.
- ラベルは軽度のアルカリ性メタノリシスに耐性があり,脂肪酸とスフィンゴシンに関連していました.
結論:
- グリコプロテインCD45は,非典型の脂質結合を通してミリストイレーションを受けます.
- この異常な改変は,CD45の細胞下部局所化または調節に役割を果たす可能性があります.
関連する概念動画
Receptor-mediated Endocytosis
Overview
Ligand Binding and Linkage
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Lipids as Anchors
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
Phosphoinositides and PIPs
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Mechanisms of Membrane Domain Formation
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...
Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

