酸素で進化する光システムIIの結晶構造は,解像度1.9 Åの解像度
Yasufumi Umena1, Keisuke Kawakami, Jian-Ren Shen
1Department of Chemistry, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka 558-8585, Japan.
Nature
|April 19, 2011
まとめ
研究者らは光システムIIの1.9 Åの結晶構造を決定し,水酸化に不可欠なマンガン・カルシウム群の詳細な配置を明らかにした. この高解像度構造は,触媒部位と潜在的な水基板チャネルを照らす.
科学分野:
- バイオケミストリー バイオケミストリー
- 構造生物学 構造生物学とは
- 光合成研究 研究 光合成研究
背景:
- 光システムII (PSII) は,光合成による水の酸化を行います.
- 以前のPSII構造 (2.93.8 Å) は,触媒水分裂センターの詳細が欠けていました.
研究 の 目的:
- 光系IIの高解像度結晶構造を決定する.
- 水分裂触媒センターの詳細な構造を解明する.
主な方法:
- X線結晶グラフィーです.
- フォトシステムIIの高解像度 (1.9 Å) 構造決定
主要な成果:
- Mn(4) CaO(5) クラスターとそのリガンドの詳細な原子構造.
- 触媒部位における5つのオキシオブリッジと4つの水分子結合の識別.
- 陽子/水/酸素のチャンネルを形成する1300以上の水分子の発見.
結論:
- 1.9 Å 構造は,光システムIIの触媒センターの前例のない詳細を提供します.
- 特定された水分子は,ダイオキシゲン形成のための基質として作用する可能性があります.
- 観測された水網は,分子輸送のチャンネルを示唆し,機能分析を助けます.
関連する概念動画
Photosystem II
59.8K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
59.8K
X-ray Crystallography
21.5K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
21.5K
Crystal Field Theory - Octahedral Complexes
28.4K
Crystal Field Theory
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...
28.4K
Protein and Protein Structure
71.4K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
A protein's shape is critical to its function. For example, an enzyme...
71.4K
Determination of Crystal Structures
135
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
135
Oxygenic Photosynthesis
991
Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate...
991


